The Effect of Auxin and Cytokinin on Black Orchid Hybrid (Coelogyne pandurata Lindley) in Vitro

Sri Hartati, Retna B.A, Brigita R.H, Ongko Cahyono

Abstract


The Orchid is an ornamental plant with high economic value due to the emergence of unique varieties. These varieties are produced from conventional breeding methods of crossing and selection. Meanwhile, the propagation of orchids through in vivo hatcheries is constrained because the seeds are too small to contain the endosperm. Tissue culture is an alternative for the propagation of orchids Efforts to propagate orchids through tissue culture is carried out by adding Growth Regulatory Substances such as auxins groups, namely NAA, IBA, IAA, and cytokinin groups, namely BA, BAP, kinetin, and TDZ to MS media, to enable the plants to take up adequate nutrients for growth. Therefore, this study aims to determine the effect of various concentrations of NAA and BA on the best ½ MS media for the growth of orchid subcultures. A completely randomized design pattern (CRD) of 2 factors with 12 treatment combinations and four replications were used, totaling 48 experimental units. The results showed that the addition of 1ppm NAA had the highest number of roots and length, while the addition of BA at 3ppm had the highest height, and the combination of 1ppm NAA and BA 3ppm treatment produced the highest number of leaves (42 sheets) and shoots. Based on the results, the addition of NAA above 3ppm followed by BA at 6ppm can produce more leaves and shoots, while NAA above 3ppm can produce higher plant height.

Keywords


Effect; NAA; BA; Coelogyne pandurate; Coelogyne rumphii.

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References


S. Hartati, Nandariyah, A. Yunus, and D. W. Djoar, “Hybridization Technique of Black Orchid (Coelogyne pandurata Lindley) to Enrich the Genetic Diversity and to Rescue the Genetic Extinction,†Bulg. J. Agric. Sci., vol. 25, no. 4, pp. 751–755, 2019, Accessed: May 12, 2021. [Online]. Available: https://www.agrojournal.org/25/04-20.html.

S. Hartati, R. B. Arniputri, L. Soliah, and O. Cahyono, “Effects of Organic Additives and Naphthalene Acetid Acid (NAA) Application on the in vitro Growth of Black Orchid Hybrid (Coelogyne pandurata Lindley).,†Bulg. J. Agric. Sci., vol. 23, no. 6, pp. 951–957, 2017, Accessed: May 12, 2021. [Online]. Available: https://www.cabdirect.org/cabdirect/abstract/20183048901.

M. Faisal, N. Ahmad, M. Anis, A. A. Alatar, and A. A. Qahtan, “Auxin-cytokinin synergism in vitro for producing genetically stable plants of Ruta graveolens using shoot tip meristems,†Saudi J. Biol. Sci., vol. 25, no. 2, pp. 273–277, Feb. 2018, doi: 10.1016/j.sjbs.2017.09.009.

I. Bamatov, E. Butsaeva, M. Arsanov, and D. Bamatov, “The modification of murashige and skoog media for efficient cultivation of gizella-5 and vsl-2 rootstocks in vitro,†in IOP Conference Series: Earth and Environmental Science, Aug. 2019, vol. 315, no. 4, p. 42015, doi: 10.1088/1755-1315/315/4/042015.

A. Kumari et al., “Plant growth regulator interactions in physiological processes for controlling plant regeneration and in vitro development of Tulbaghia simmleri,†J. Plant Physiol., vol. 223, pp. 65–71, Apr. 2018, doi: 10.1016/j.jplph.2018.01.005.

R. Mastuti, A. Munawarti, and E. R. Firdiana, “The combination effect of auxin and cytokinin on in vitro callus formation of Physalis angulata L. - A medicinal plant,†in AIP Conference Proceedings, Nov. 2017, vol. 1908, no. 1, p. 40006, doi: 10.1063/1.5012721.

S. M. Haque and B. Ghosh, “Regeneration of Cytologically Stable Plants Through Dedifferentiation, Redifferentiation, and Artificial Seeds in Spathoglottis plicata Blume. (Orchidaceae),†Hortic. Plant J., vol. 3, no. 5, pp. 199–208, Sep. 2017, doi: 10.1016/j.hpj.2017.10.002.

N. P. Huy et al., “In vitro polyploid induction of Paphiopedilum villosum using colchicine,†Sci. Hortic. (Amsterdam)., vol. 252, pp. 283–290, Jun. 2019, doi: 10.1016/j.scienta.2019.03.063.

P. Bhattacharyya, V. Kumar, J. Grúz, K. Doležal, and J. Van Staden, “Deciphering the phenolic acid reserves and antioxidant activity within the protocorm like bodies of Ansellia africana: A vulnerable medicinal orchid,†Ind. Crops Prod., vol. 135, pp. 21–29, Sep. 2019, doi: 10.1016/j.indcrop.2019.03.024.

H. K. Chauhan, A. K. Bisht, I. D. Bhatt, and A. Bhatt, “Protocol for vegetative propagation of Trillium govanianum Wall ex D. Don,†J. Appl. Res. Med. Aromat. Plants, vol. 16, p. 100233, Mar. 2020, doi: 10.1016/j.jarmap.2019.100233.

M. Asa and B. Kaviani, “In vitro propagation of orchid Phalaenopsis amabilis (L.) Blume var. Jawa,†Iran. J. Plant Physiol., vol. 10, no. 2, pp. 3113–3123, Apr. 2020, doi: 10.22034/IJPP.2020.672571.

R. V. Garvita and Sahromi, “Plant Regeneration Through Direct Somatic Embryogenesis from Leaf Explants of Paraphalaenopsis Labukensis P. S. Shim,†in IOP Conference Series: Earth and Environmental Science, Dec. 2019, vol. 394, no. 1, p. 012053, doi: 10.1088/1755-1315/394/1/012053.

L. A. Rodrigues et al., “Propagação in vitro de Cyrtopodium saintlegerianum rchb. F. (orchidaceae), uma orquídea nativa do cerrado Brasileiro,†Crop Breed. Appl. Biotechnol., vol. 15, no. 1, pp. 10–17, 2015, doi: 10.1590/1984-70332015v15n1a2.

M. C. R. Juras, J. Jorge, R. Pescador, W. De Melo Ferreira, V. Tamaki, and R. M. Suzuki, “In vitro culture and acclimatization of Cattleya xanthina (Orchidaceae), an endangered orchid of the Brazilian Atlantic Rainforest,†Rodriguesia, vol. 70, 2019, doi: 10.1590/2175-7860201970014.

F. A. Ozdemir, “Effects of 6-benzylaminopurine and α-naphthalene acetic acid on micropropagation from ten days old cotyledon nodes of Mentha spicata subsp. spicata received for.,†Rom. Biotechnol. Lett., vol. 22, no. 3, pp. 12554–12559, 2017, Accessed: May 12, 2021. [Online]. Available: https://www.cabdirect.org/cabdirect/abstract/20183041025.

C. R. Deb and H. Y. Jakha, “Factors affecting asymbiotic immature seed culture and in vitro propagation of Paphiopedilum insigne (wall. Ex. Lindl.) pfitzer –a horticultural important vulnerable orchid,†Plant Cell Biotechnol. Mol. Biol., vol. 21, no. 15–16, pp. 129–141, Jul. 2020, Accessed: May 12, 2021. [Online]. Available: https://ikprress.org/index.php/PCBMB/article/view/5178.

S. M. Haque, S. Kazuhiko, and S. J. Nahar, “Combination treatment of 6-benzylaminopurine (BA) and hyaluronic acid (HA) on plb culture of Cymbidium spp. In vitro,†Am. J. Agric. Environ. Sci., vol. 16, no. 7, pp. 1335–1340, 2016, doi: 10.5829/idosi.aejaes.2016.16.7.12948.

P. Gaurav and P. Bijaya, “In vitro seed germination and seedling development of the orchid Coelogyne stricta (D. Don) Schltr.,†African J. Biotechnol., vol. 15, no. 5, pp. 105–109, Feb. 2016, doi: 10.5897/ajb2015.14870.

N. P. Devi, B. Lisipriya, and N. Bai, “Asymbiotic seed germination and mass multiplication of Taprobanea spathulata (L.) Christenson (Asparagales: Orchidaceae): a medicinally important epiphytic orchid,†Brazilian J. Biol. Sci., vol. 2, no. 4, pp. 271–286, Dec. 2015, Accessed: May 12, 2021. [Online]. Available: http://revista.rebibio.net.

S. Parthibhan, M. V. Rao, and T. Senthil Kumar, “In vitro regeneration from protocorms in Dendrobium aqueum Lindley - An imperiled orchid,†J. Genet. Eng. Biotechnol., vol. 13, no. 2, pp. 227–233, Dec. 2015, doi: 10.1016/j.jgeb.2015.07.001.

S. Konar, Adhikari S, Karmakar J, Ray A, and Bandyopadhyay TK, “Evaluation of subculture ages on organogenic response from root callus and spar based genetic fidelity assesment in the regenerant of Hibiscus sabdariffa L. †J. Industrial Crops & Products, vol. 135, no. 1, pp. 321-329, 2019.




DOI: http://dx.doi.org/10.18517/ijaseit.12.3.14192

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