Antioxidant Activity of Melinjo Ketan (Gnetum gnemon L., ‘Ketan’) Seed Extract at Various Ripening Stages and Ethanol Solvent Concentration

Bambang Kunarto, Sutardi Sutardi, - Supriyanto, Chairil Anwar

Abstract


Melinjo seeds (Gnetum gnemon L.) have the potency as antioxidants due to their bioactive compounds such as polyphenols, flavonoids, tannins, and resveratrol. The study was conducted to determine the effect of the ripening stages of Melinjo ketan variety seeds and ethanol solvent concentration on total of phenolic, flavonoid also resveratrol content, and antioxidant activity of Melinjo seed extract. The ripening stages of Melinjo seeds used were immature, semi-mature and mature. The ethanol solvent concentrations were 20%, 50% and 80%. Samples of dried melinjo seeds were macerated in ethanol (1: 5) at room temperature for 2 days. The extract was filtrated, evaporated and freeze-dried. This study reported that the ripening stages of melinjo seeds and ethanol solvent concentrations hadeffect on the total of phenolic, flavonoid, resveratrol content, and antioxidant activity of melinjo seed extractsignificantly (p<0,05). Mature melinjo seeds macerated using 50% ethanol solvent produced extract with the highest antioxidant activity. It had the total phenolic content of 7.033 ± 0.06 mg GAE/g, total flavonoids content of 335.04 ± 1.32 mg CE/100 g, resveratrol content of 1.55 ± 0.3%, DPPH radical inhibition of 66.07 ± 0.38%, and reducing power (80.26 ± 0.06%).These studies showed that the antioxidant effect of Melinjo ketan extract is influenced by the level of maturity and solvent concentrations of ethanol that can be an alternative of antioxidant sources. It is recommended to use Melinjo ketan seeds macerated with 50% ethanol solvent. 

Keywords


antioxidant; melinjo ketan; resveratrol; ripening stage.

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References


T. Tatefuji, M. Yanagihara, S. Fukushima, and K. Hashimoto, “Safety assessment of melinjo (Gnetum gnemon L.) seed extract: Acute and sub chronic toxicity studies,” Food and Chemical Toxicology, vol 67, pp 230-235, 2014.

T. A. Siswoyo, “Physicochemical Characteristics Of Melinjo (Gnetum gnemon) Starch-Lipid,” Jurnal Ilmu Dasar, Vol. 5, No. 2, pp. 97-102, 2004.

W. Guo, A. Li, Z. Jia, Y. Yuan, H. Dai, and H. Li, “Transferrin modified PEG-PLA-resveratrol conjugates: in vitro and in vivo studies for glioma,” Eur. J. Pharmacol., Vol. 718, pp. 41- 47, 2013.

M. Elshaer, Y. Chen, X. J. Wang, and X. Tang, “Resveratrol: An overview of its anti-cancer mechanisms,” Life Sci., Vol. 207, pp. 340-349, 2018

C.W. Lee, F. L. Yen, H. W. Huang, T. H. Wu, H. H. Ko, W. S. Tzeng, and C. C. Lin, ”Resveratrol nanoparticle system improves dissolution properties and enhances the hepatoprotective effect of resveratrol through antioxidant and anti-inflammatory pathways,” J. Agric. Food Chem., Vol. 60, No. 18, pp. 4662-4671, 2012.

P. E. B. Ferreira, E. J. Beraldi, S. C. Borges, M. R. M. Natali, and N. C. Buttow, “Resveratrol promotes neuroprotection and attenuates oxidative and nitrosative stress in the small intestine in diabetic rats,” Biomed Pharmacother, Vol. 105, pp. 724-733, 2018.

Nakata, and H. H. Inoue, “Resveratrol and Cardiovascular Disease,” Current Nutrition Reports, Vol. 3, No. 3, pp. 163-169, 2014.

U. Svajger, and M. Jeras, “Anti-inflammatory effects of resveratrol and its potential use in therapy of immune-mediated diseases,” Int. Rev. Immunol., Vol. 31, No. 3, pp. 202-222, 2012.

C. C. Chang, K. Lin, K. Peng, Y. Day, and L. Hung, ”Resveratrol exerts anti-obesity effects in high-fat diet obese mice and displays differential dosage effects on cytotoxicity, differentiation, and lipolysis in 3T3-L1 cells,” Endocrine Journal, Vol. 63, No. 2, pp. 169-178, 2016.

M. M. M. Hussein, and M. K. Mahfouz, ”Effect of resveratrol and rosuvastatin on experimental diabetic nephropathy in rats,” Biomedicine & Pharmacotherapy, Vol. 82, pp. 685-692, 2016.

C. Iuga, J. R. Alvarez-Idaboy, and N. Russo, "Antioxidant activity of trans-resveratrol toward hydroxyl and hydroperoxyl radicals: a quantum chemical and computational kinetics study," The Journal of organic chemistry, 77 (8), pp. 3868-3877, 2012

J. Gerszon, A. Rodacka, and M. Puchała, "Antioxidant properties of resveratrol and its protective effects in neurodegenerative diseases,." Advances in Cell Biology, 4 (2), pp. 97-117, 2014.

G. Davidov-Pardo, and D. J. McClements, D. J., “Resveratrol encapsulation: Designing delivery systems to overcome solubility, stability and bioavailability issues,” Trends in Food Science and Technology, Vol. 20, pp. 1-16, 2014.

M. Aminzare, M. Hashemi, E. Ansarian, M. Bimkar, H. H. Azar, M. R. Mehrasbi, S. Daneshamooz, M. Raeisi, B. Jannat, and A. Afshari, "Using natural antioxidants in meat and meat products as preservatives: A review," Advances in Animal and Veterinary Sciences 7, pp. 417-426, 2019.

S. A. Rege, and S. A. Momin, "Synergistic Antioxidant Activity of Clove Oleoresin with Capsicum Oleoresin and Kalonji Seeds Extract in Sunflower Oil.," International Journal of Nutritional Disorders & Therapy, Vol. 1. 2018.

X. Dong, Y. Hu, Y. Li, and Z. Zhiqin, 2019. “The maturity degree, phenolic compounds and antioxidant activity ofEureka lemon [Citrus limon (L.) Burm. f.]: A negative correlation between total phenolic content, antioxidant capacity, and soluble solid content,” Scientia Horticulturae, Vol. 243, pp.281–289, 2019.

Y. Zhou, P. Su, H. Yin, Z.Dong, L Yang, and C. Yuan, “Effects of different harvest times on the maturity of polyphenols in two red wine grape cultivars (Vitis vinifera L.) in Qingtongxia (China),” S. Afr. J. Enol. Vitic., Vol. 40, No. 1, 2018.

Z. Zhang, D. Li, L. Wang, N. Ozkan, X. D. Chen, Z. Mao, and H. Yang, “Optimization of ethanol-water extraction of lignans from flaxseed,” Separation and Purification Technology, Vol. 57, No. 1, pp. 17-24, 2007.

D.E. Blank, D.E., D. Justen, S. Fraga, C. R. Peixoto, and N. F. de Moura, “Chemical Composition and Antioxidant Activity of Bunchosia glandulifera Fruit at Different Ripening Stages,” Food and Nutrition Sciences, 9(10), pp.1147-1159, 2018.

M. Oliveira, P. Bicudo, and R. H. Ribani, “Trust β-anthocyanins, phenolic acids and antioxidant properties of juçara fruits (Euterpe edulis M.) along the on-tree ripening process,” Plant Foods Hum. Nutr., Vol. 69, pp. 142 – 147, 2014.

E. Kato, Y. Tokunaga, and F. Sakan, “Stilbenoids isolated from the seeds of melinjo (Gnetum gnemon L.) and their biological activity,” J. Agric. Food Chem., 57(6), pp. 2544 – 2549, 2009.

V. Dewanto, X. Wu, K.K. Adom, and R. H. Liu, “Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity,” Journal of agricultural and food chemistry, 50(10), pp. 3010-3014, 2002.

J. Zhishen, T. Mengcheng, and W. Jianming, “The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals,” Food Chemistry, Vol. 64, pp. 555 - 559. 1999.

R. Bhat, and B. N. Yahya, “Evaluating melinjau (Gnetum gnemon L.) seed flour quality as a base for development of novel food products and food formulations,” Food Chemistry, Vol. 156, pp. 42 - 49. 2014.

Y. Liu, L. Nan, J. Liu, H. Yan, D. Zhang, and X. Han, “Isolation and identification of resveratrol producing endophytes from wine grape Cabernet Sauvignon,” Springer Plus, Vol. 5, pp. 1-13, 2016.

I. Savic-gajic, I.M. Savic, V. D. Nikolic, B. Nikolic, M. M. Popsavin, and S. J. Rakic, “The improvement of photostability and antioxidant activity of trans-resveratrol by cyclodextrins,” Advanced Technologies, Vol. 6, No. 2, pp. 18-25, 2017.

N. Ozsoy, A. Can, R. Yanardag, and N. Akev, “Antioxidant activity of Smilax excelsa L. leaf extracts,” Food Chemistry, Vol. 110, pp. 571–583, 2008.

J. Yang, R. Gadi, and Talene Thomson, “Antioxidant capacity, total phenols, and ascorbic acid content of noni (Morinda citrifolia) fruits and leaves at various stages of maturity,” Micronesica, Vol. 41, No. 2, pp.167–176, 2011.

T. Mahmood, F. Anwar, M. Abbas, and N. Saari, “Effect of maturity on phenolics (phenolic acids and flavonoids) profile of strawberry cultivars and mulberry species from Pakistan,” Int. J. Mol. Sci., Vol. 13, pp. 4591-4607, 2012.

I. B. Rebey, W. A. Wannes, S. B. Kaab, S. Bourgou, M. S. Tounsi, R. Ksouri, and M. L. Fauconnier, "Bioactive compounds and antioxidant activity of Pimpinella anisum L. accessions at different ripening stages, " Scientia Horticulturae 246, pp 453-461, 2019.

D. Wazir, S. Ahmad, R. Muse, M. Mahmood, and M. Y. Shukor, “Antioxidant activities of different parts of Gnetum gnemon L.,” Journal of Plant Biochemistry and Biotechnology, Vol. 20, No. 2, pp. 234 – 240, 2011.

I. Bettaieb-Rebey, S. Kefi, S. Bourgou, I. Ouerghemmi, R. Ksouri, M.S. Tounsi, and B. Marzouk, “Ripening stage and extraction method effects on physical properties, polyphenol composition and antioxidant activities of cumin (Cuminum cyminum L.) seeds,” Plant Foods for Human Nutrition, Vol. 69, pp. 358-364, 2014

M. Dûma, I. Alsiòa, L. Dubova, and L. Erdberga, “Bioactive compounds in tomatoes at different stages of maturity,” Proceedings Of The Latvian Academy Of Sciences. Section B, Vol. 72, No. 2, pp. 85–90. 2018. DOI: 10.2478/prolas-2018-0014.

M. M. Hasan, and H. Bae, “An overview of stress induced resveratrol synthesis in grapes: perspectives for resveratrol-enriched grape product,” Molecules, Vol. 22, pp. 294–312. 2017

Y. S. Wang, C. Chen, Y. Chien, Wang, and P. Chen, “Resveratrol Content in Strawberry Fruit Is Affected by Preharvest Conditions,” J. Agric. Food Chem., Vol. 55, pp. 8269–8274, 2007.

C. Guo, J. Yang, J. Wei, Y. Li, J. Xu, and Y. Jiang, Y, “Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay,” Nutrition Research, Vol. 23, pp. 1719 -1726, 2003.

Y. Liu, Y. Qi, X. Chen, H. He, Z. Liu, Z. Zhanga, Y. Ren and X. Ren, “Phenolic compounds and antioxidant activity in red- and in green-fleshed kiwifruits,” Food Research International, Vol. 116, pp. 291-301, 2019.

M. Atanassova, S. Georgieva, and K. Ivancheva, “Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medicinal herbs,” Journal of The University of Chemical Technology and Metallurgy, Vol. 46, No. 1, pp. 81-88, 2011.

M. Mahmoudi, F. Boughalleb, M. Mabrouk, N. Tlili, D. Potter, R. Abdellaoui, and N. Nasri, “Chemical analysis of the antioxidants from the aerial parts of wild Polygonum equisetiforme from Tunisia,” Food Bioscience, Vol. 29, pp.24-39. 2019.

M. Li, P. W. Pare, J. Zhang, T. Kang, Z. Zhang, D. Yang, K. Wang, and Hua, “Antioxidant capacity connection with phenolic and flavonoid content in Chinese medicinal herbs,” Rec. Nat. Prod., Vol. 12, No. 3, pp. 239-250, 2018.

I. Iliya, Z. Ali, T. Tanaka, M. Iinuma, M. Furusawa, K. Nakayama, J. Murata, D. Darnaedi, N. Matsuura, and M. Ubukata, M., “Stilbene derivatives from Gnetum gnemon Linn.,” Phytochemistry, Vol. 62, pp. 601-606, 2003.




DOI: http://dx.doi.org/10.18517/ijaseit.9.4.9376

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