Membrane Performance of Micro and Ultrafiltration on Folic Acid Separation from Dent Corn (Zea mays var. indentata) Hydrolyzed by Rhizopus oligosporus-C1
Performance of microfiltration (MF) and ultrafiltration (UF) membranes to separate of folic acid from corn (Zea mays var. indentata) hydrolysate equipped in stirred filtration cell (SFC) mode were performed as a reference guide toward semi pilot scale. Separation on hydrolysate suspension of yellow dent corn (HSYCD) and hydrolysate suspension of white dent corn (HSWCD) as a results of hydrolysis of protease enzyme of Rhizopus oligosporus strain-C1 0.025 % and 0.075 % (w/w, dissolved protein) at pH 5 and 30 °C for 24 hours was conducted by MF (0.45 µm) and UF (100000 MWCO) membranes at stirrer rotation speed 400 rpm and trans-membrane pressure (TMP) of 20, 30 and 40 psia for 30 minutes. The experimental result showed that based on optimization of fluxes, the best performances of MF and UF membranes on HSYDC and HSWDC were achieved at TMP 40 psia and gave fluxes of 0.0534 and 0.0508 mL/cm2.min., respectively. In these process conditions, it take place an increase of folic acid in concentrates of HSYDC and HSWDC compared with before process (feed). Identification of molecular weight (MW) on folic acid from HSYDC and HSWDC displayed dominant folic acid monomer at T2.7, and T2.4 and T2.89. This matter showed that commodity of yellow dent corn has more potential use as source of folic acid compared with white dent corn at both similar condition of hydrolysis and separation and/or purification processes.
R.V. Reis and A.L. Zydney, “Bioprocess membrane technology,” Journal of Membrane Science. 297, pp.16.2007.
B. Bhayani, I.R. Yasarla and B.V. Ramarao, “Membrane filtration of lignocellulosic hydrolysates : Comparison of crossflow and dead-end microfiltration,” Center for Energy Initiatives (CEI), American Institute of Chemical Engineers (AlChE). October 18, 2011.
FDA News Release, FDA approves folic acid fortification of corn masa flour. April 14, 2016.
J.S. Phale and Y.J. Chendake, “Polysulfone based Ultrafiltration Membrane Preparation by Phase Inversion : Parameter Optimization,” International Journal of Science and Research (IJSR), June 2016.
I. Ahmed, “High performance UF polyester - polysulfone membrane,” [Ph.D. Thesis]. University Technology Malaysia, Malaysia. 2009.
L.L. Ngoc and S.P. Nunes, “Materials and membrane technologies for water and energy sustainability,” Journal of Sustainable Materials and Technologies, 7, pp.5. 2016.
NIST. National Institute of Standards and Technology (NIST) Chemistry WebBook, SRD 69, U.S.A. 2017.
O.J. Olsen, “Operating Manual of DSS LabUnit M20,” Danish Separation Systems AS, DK-4900 Nakskov, Denmark. January 2000.
A.L. Zydney, High Performance Ultrafiltration Membranes : Pore Geometry and Charge Effects, in : S.T. Oyama and S.M. Stagg-Williams (Eds.). Membrane Science and Technology, Elsevier, Amsterdam, Vol. 14, pp.333. 2011.
P. Kumar, N. Sharma, R. Ranjan, S. Kumar, Z.F. Bhat and D.K. Jeong, “Perspective of Membrane Technology in Dairy Industry : A Review,” Asian-Australasian Journal of Animal Sciences (AJAS), 26 (9), pp.1347. 2013. DOI: http://dx.doi.org/10. 5713/ajas.2013.13082.
W.J. Koros, Y.H. Ma and T. Shimidzu, T. “Terminology for membranes and membrane process (IUPAC Recommendations 1996),” Journal of Membrane Science, 120, pp.149. 1996.
D. Onggo, “General Principles in Electrospray Mass Spectrometry : A New Technique in Mass Spectral Analysis,” Journal of Mass Spectrum, Vol. 3, No. 2, pp.115. Accessed at February 3, 2013.
MILLIPORE, “Catalogue and Product Information of Stirred Ultrafiltration Cell,” Amicon Bioseparation, MILLIPORE, Bedford, U. S. A. www.millipore.com. 2008.
A.O.A.C., “Official Methods of Analysis,” Association of Official Analytical Chemists International, 20th. Edition, Washington D. C. 2016.
W. Ruengsitagoon and N. Hattanat, Simple spectrophotometric method for determination of folic acid. The 4th Annual Northeast Pharmacy Research Conference, Thailand. 2012.
P. Eichhorn and T.P. Knepper, “Electrospray ionization mass spectrometric studies on the amphoteric surfactant cocamidopropylbetaine,” Journal of Mass Spectrum, 36 (6), pp.677 – 84. June 2001. DOI : 10.1002/jms.170.
A. Susilowati, Y.M. Iskandar, H. Melanie, Y. Maryati, P.D. Lotulung and D.G. Aryani, “Pengembangan konsentrat sayuran hijau dan kacang-kacangan terfermentasi pada jagung (Zea mays L.) pramasak sebagai sumber asam folat untuk formula pangan pintar,” Laporan Hasil Penelitian, Program Tematik, Kedeputian IPT, Pusat Penelitian Kimia – LIPI, Kawasan PUSPIPTEK, Serpong, Tangerang Selatan. 2016.
N.A. Rodríguez-Martínez, M.G. Salazar-García, B.R. Wong, A.R. Islas-Rubio, L.C. Platt-Lucero, Morales-Rosas, R. Marquez-Melendez and F. Martínez-Bustos, “Effect of Malting and Nixtamalization Processes on the Physicochemical Properties of Instant Extruded Corn Flour and Tortilla Quality,” Plant Foods Hum. Nutr. 70, pp.275. Published online : 10 June 2015. Springer Science+Business Media New York. DOI : 10.1007/s 11130-015-0490-9. CrossMark.
A.S. Grandison and M.J. Lewis, Separation Processes in the Food and Biotechnology Industries : Principles and Applications. Technomic Publishing Company Inc., Lancaster, Pennsylvania, U.S.A. First publication. 1996.
D. Mancinelli and C. Hallé, “Nano-Filtration and Ultra-Filtration Ceramic Membranes for Food Processing : A Mini Review,” Journal of Membrane Science and Technology, Vol. 5, Issue 2. 2015. http://dx.doi.org/10. 4172/2155-9589.1000140.
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