Sustainable Design of Biorefinery Processes on Cocoa Pod: Optimization of Pectin Extraction Process with Variations of pH, Temperature, and Time

- Desniorita, Novizar Nazir, - Novelina, Kesuma Sayuti


Pectin is a polysaccharide compound, which is a D-galacturonic acid polymer that is connected by α-1-4 glycosidic bonds. Pectin can be extracted from the cocoa pod. This research is part of research on the Sustainable Design of biorefinery processes of cocoa pod.  The aim of this research were to extract pectin from cocoa pod by optimizing pH, temperature and time. The response from this study was the yield, pectin content and methoxyl content. This research used the Surface Response Methodology (RSM) with  the center point obtained was pH 2.5, temperature 95 ° C and time was 3 hours.  The treatment consisted of pH, temperature, time, and the expected response was the results of pectin, pectin content, and methoxyl content. The result showed that the pH, temperature and extraction time of cocoa pod had significant effect to the yield of pectin, pectin content and methoxyl content. The optimum conditions for the extraction of pectin were pH of 2, temperature of 95 °C and extraction time of 3 hours with a predicted yield of pectin of 2.33%, pectin content of 44.39% and methoxyl content of 7.07%. The functional groups between the standard pectin and the cocoa pod  pectin are not significantly different. They have the same variety of functional groups. This data supports that the extracted pectin has the same structure with standard pectin which is shown in the IR spectrum of of FTIR test.


pectin; extraction; cocoa pod; optimization; biorefinery.

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F. Fioresi et al., “Chemical modification of the cocoa shell surface using diazonium salts,” J. Colloid Interface Sci., vol. 494, pp. 92–97, 2017.

Y. Chen, J. G. Zhang, H. J. Sun, and Z. J. Wei, “Pectin from Abelmoschus esculentus: Optimization of extraction and rheological properties,” Int. J. Biol. Macromol., vol. 70, pp. 498–505, 2014.

S. Khanahmadi, F. Yusof, H. Chyuan Ong, A. Amid, and H. Shah, “Cocoa pod husk: A new source of CLEA-lipase for preparation of low-cost biodiesel: An optimized process,” J. Biotechnol., vol. 231, pp. 95–105, 2016.

M. D. G. De Luna, Murniati, W. Budianta, K. K. P. Rivera, and R. O. Arazo, “Removal of sodium diclofenac from aqueous solution by adsorbents derived from cocoa pod husks,” J. Environ. Chem. Eng., vol. 5, no. 2, pp. 1465–1474, 2017.

P. Judith et al., “Food Hydrocolloids Edible fi lms from pectin: Physical-mechanical and antimicrobial properties - A review,” vol. 35, 2014.

R. M. Zaid, P. Mishra, S. Tabassum, Z. A. Wahid, and A. M. M. Sakinah, “High methoxyl pectin extracts from Hylocereus polyrhizus’s peels: Extraction kinetics and thermodynamic studies,” Int. J. Biol. Macromol., 2019.

F. Priyangini, S. G. Walde, and R. Chidambaram, “Extraction optimization of pectin from cocoa pod husks (Theobroma cacao L.) with ascorbic acid using response surface methodology,” Carbohydr. Polym., vol. 202, pp. 497–503, 2018.

A. M. Idrovo Encalada et al., “High-power ultrasound pretreatment for efficient extraction of fractions enriched in pectins and antioxidants from discarded carrots (Daucus carota L.),” J. Food Eng., vol. 256, pp. 28–36, 2019.

B. Utomo, A. A. Prawoto, S. Bonnet, A. Bangviwat, and S. H. Gheewala, “Environmental performance of cocoa production from monoculture and agroforestry systems in Indonesia,” J. Clean. Prod., vol. 134, no. Part B, pp. 583–591, 2016.

S. S. Hosseini, F. Khodaiyan, and M. S. Yarmand, “Aqueous extraction of pectin from sour orange peel and its preliminary physicochemical properties,” Int. J. Biol. Macromol., vol. 82, pp. 920–926, 2016.

P. H. F. Pereira et al., “Pectin extraction from pomegranate peels with citric acid,” Int. J. Biol. Macromol., vol. 88, pp. 373–379, 2016.

C. Colodel, L. C. Vriesmann, R. F. Teófilo, and C. L. de Oliveira Petkowicz, “Extraction of pectin from ponkan (Citrus reticulata Blanco cv. Ponkan) peel: Optimization and structural characterization,” Int. J. Biol. Macromol., vol. 117, pp. 385–391, 2018.

R. F. Caroço, B. Kim, P. A. Santacoloma, J. Abildskov, J. H. Lee, and J. K. Huusom, “Analysis and model-based optimization of a pectin extraction process,” J. Food Eng., vol. 244, pp. 159–169, 2019.

S. Y. Chan and W. S. Choo, “Effect of extraction conditions on the yield and chemical properties of pectin from cocoa husks,” Food Chem., vol. 141, no. 4, pp. 3752–3758, 2013.

A. A. Sundarraj, R. Thottiam Vasudevan, and G. Sriramulu, “Optimized extraction and characterization of pectin from jackfruit (Artocarpus integer) wastes using response surface methodology,” Int. J. Biol. Macromol., vol. 106, pp. 698–703, 2018.

C. Li, Q. Xiao, Y. Tang, and L. Li, “A method integrating Taguchi, RSM and MOPSO to CNC machining parameters optimization for energy saving,” J. Clean. Prod., vol. 135, pp. 263–275, 2016.

J. Lu, J. Li, R. Jin, S. Li, J. Yi, and J. Huang, “Extraction and characterization of pectin from Premna microphylla Turcz leaves,” Int. J. Biol. Macromol., vol. 131, pp. 323–328, 2019.

S. Q. Liew, W. H. Teoh, C. K. Tan, R. Yusoff, and G. C. Ngoh, “Subcritical water extraction of low methoxyl pectin from pomelo (Citrus grandis (L.) Osbeck) peels,” Int. J. Biol. Macromol., vol. 116, pp. 128–135, 2018.

Z. Raji, F. Khodaiyan, K. Rezaei, H. Kiani, and S. S. Hosseini, “Extraction optimization and physicochemical properties of pectin from melon peel,” Int. J. Biol. Macromol., vol. 98, pp. 709–716, 2017.

M. Abid, C. M. G. C. Renard, A. A. Watrelot, I. Fendri, H. Attia, and M. A. Ayadi, “Yield and composition of pectin extracted from Tunisian pomegranate peel,” Int. J. Biol. Macromol., vol. 93, pp. 186–194, 2016.

J. Zheng, R. Zeng, J. Kan, and F. Zhang, “Effects of ultrasonic treatment on gel rheological properties and gel formation of high-methoxyl pectin,” J. Food Eng., vol. 231, pp. 83–90, 2018.

W. Wang et al., “Applications of power ultrasound in oriented modification and degradation of pectin: A review,” J. Food Eng., vol. 234, pp. 98–107, 2018.

X. Zhai, C. Zhu, Y. Li, Y. Zhang, Z. Duan, and X. Yang, “Optimization for pectinase-assisted extraction of polysaccharides from pomegranate peel with chemical composition and antioxidant activity,” Int. J. Biol. Macromol., vol. 109, pp. 244–253, 2018.

I. G. Moorthy, J. P. Maran, S. M. Surya, S. Naganyashree, and C. S. Shivamathi, “Response surface optimization of ultrasound assisted extraction of pectin from pomegranate peel,” Int. J. Biol. Macromol., vol. 72, pp. 1323–1328, 2015.

C. N. Tejada-tovar and A. Villabona-ortiz, “Evaluation of Theobroma cacao Waste Performance in Nickel Removal (II) in Continuous System*,” Int. J. ChemTech Res., vol. 11, no. 05, pp. 186–194, 2018.



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