Stimulating Activity on Human Lymphocytes in vitro of Nori like Product (Geluring) Made from Gelidium sp. and Ulva lactuca Seaweeds

- Erniati, Fransiska Zakaria Rungkat, Endang Prangdimurti, Dede Robiatul Adawiyah, Bambang Pontjo Priosoeryanto, Nurul Huda

Abstract


Seaweed has been reported to contain bioactive compounds that have an immunomodulatory activity, such as stimulating activity on human lymphocytes. Nori like the product from the mixture of Gelidium sp. and Ulva lactuca seaweed in this research named “geluring†may improve the immune system. This research is aimed to determine the stimulating activity of geluring by observing proliferation activity and interleukin-2 (IL-2) production of human lymphocyte by in vitro method. Two types of geluring were prepared, that were P1 (unseasoned geluring) and P2 (seasoned geluring) according to the commercial nori process with some modification. Gelurings were extracted with water and the extracts were added into lymphocyte cultures with various concentrations. The results showed that extracts of P1 and P2 gelurings could stimulate lymphocyte proliferation and IL-2 production significantly (P<0.05) compared with the stimulations demonstrated by the  cultures stimulated with PHA, RPMI, and extracts of unprocessed dried Ulva lactuca (UP0) but not significantly as compare to cultures added with unprocessed dried Gelidium sp.(GP0). Moreover, P2 geluring showed stimulation of lymphocyte proliferation and IL-2 production higher than P1 geluring and those of the control cultures. There was a positive correlation of proliferative activity with IL-2 production of the lymphocytes. The stimulation of lymphocyte proliferation and IL-2 production by P1 and P2 gelurings was significantly influenced by the concentration of the extracts. The concentration of 2.66 mg/ml culture showed the highest proliferation and IL-2 production of the cells. Base on this research, it can be concluded that geluring products made from the mixture of Gelidium sp. and Ulva lactuca stimulate the activity of lymphocytes, which indicates the potential to be used as a health food to improve the immune system.

Keywords


immune system; interleukin-2; lymphocytes proliferation; geluring; seaweed.

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References


F.R. Zakaria, B.P. Priosoeryanto, Erniati and Sajida, “Characteristic of the nori from mixed Ulva lactuca and Eucheuma cottoni seaweeds,†Journal of Postharvest, Fisheries and Marine Biotechnology, vol. 12(1), pp. 23–30, 2017.

Erniati, F.R. Zakaria, E. Prangdimurti, D.R.Adawiyah, B.P. Priosoeryantoand N. Huda, “Chemical evaluation of nori-like product (geluring) made from the mixture of Gelidium sp. and Ulva lactuca seaweeds,†Curent Research Nutrition and Food Science,vol. 6(3), pp. 664-671, 2018.

I. Fernández-Segovia, M.J. Lerma-García, A. Fuentes and J.M. Barat, “Characterization of Spanish powdered seaweeds: Composition, antioxidant capacity and technological propertie,†Food Research International, vol. 111, pp. 212-219, 2018.

T. Barahona, M.V. Encinas, M. Imarai, A. Mansilla, B. Matsuhiro, R. Torres and B. Valenzuela, “Bioactive polysaccharides from marine algae,†Journal Bioactive Carbohydrat and Diettary Fibre, vol. 4, pp. 125 – 138, 2014.

R. Othman, N.A. Amin, M.S.A. Sani, N.A. Fadzillah and M.A. Jamaludin, “Carotenoid and chlorophyll profile in Five Species of Malaysian Seaweed as Potential Halal Active Pharmaceutical Ingredient (API),†International Journal on Advanced Science, Engineering, Information Technology, vol. 8(4-2), pp. 1610-1616, 2018.

U.S. Patil, A.V. Jaydeokar and D.D. Bandawane, “Immunomodulators: A pharmacological review,†Internationa Jornal of Pharmcy and Pharmaceutical Sciencei., vol. 4 (Suppl 1), pp. 30-36, 2012.

P.K.M. Nagarathna, K. Reena, R. Sriram and J. Wesley, “Review on immunomodulation and immunomodulatory activity of some herbal plants,†International Journal of Pharmaceutical. Science. Review and Research, vol. 22(1), pp. 223-230, 2013.

M.C. Kang, W.A.J.P. Wijesinghe, S.H. Lee, S.M. Kang, S.C.Ko,X.Yang, N. Kang, B.T. Jeon, J. Kim, D.H. Lee and Y.J. Jeon, “Dieckol isolated from brown seaweed Ecklonia cava attenuates type II diabetes in db/db mouse model,†Food and Chemical Toxicology, vol. 53, pp. 294–298. 2013.

K. Chen, J.J. Ríos, A. Perez-Galvez and M. Roca, “Comprehensive chlorophyll composition in the main edible seaweeds,â€. Food Chemistry, vol. 228, pp. 625-633, 2017.

R. Haryatfrehni, S.C. Dewi, A. Meilianda, S. Rahmawati and I.Z.R. Sari, “Preliminary study the potency of macroalgae in Yogyakarta: extraction and analysis of algal pigments from common gunung kidul seaweeds,†Procedia Chemistry, vol. 14, pp. 373-380, 2015.

N. Peasura, N. Laohakunjit, O. Kerdchoechuen, P. Vongsawasdi and L.K. Chao, “Assessment of biochemical and immunomodulatory activity of sulphated polysaccharides from Ulva intestinalis,†International Journal of Biological Macromolecules, vol. 91, pp. 269-277, 2016.

F.R. Zakaria, E. Prangdimurti, G.A.K.D. Puspawati, R. Thahir and Suismono, “Sorgum (Sorghum bicolor L Moench) based diet improves lymphocytes proliferation and antioxidant capacity of rat liver,†Food, vol. 20 (3), pp. 209-222, 2011.

K. Zailanie, H. Kartikaningsih and U. Kalsum, “Effect of Sargassum filipendula fucoxanthin against HeLa cell and lymphocyte proliferation,†Journal Life Science Biomedical, vol. 5(2), pp. 53-59, 2015.

S.L. Gaffen and K.D. Liu KD, “Overview of interleukin-2 function, production and clinical applications,†Cytokine, vol. 28, pp. 109-123, 2004.

M.P. Capobianco, G.C. Cassiono, A.A.C. Furini, L.M.S. Melo, C.R.B. Domingos and R.L.D. Machado, “Human interleukin 2 (IL-2) promotion of immune regulation and clinical outcomes: A Review,†Journal Cytokine Biology, vol. 1(2), pp. 1-4, 2016.

G. Ahn, S.J. Bing, S.M. Kang, W.W. Lee, S.H. Lee, H. Matsuda, A. Tanaka, I.H. Cho, Y.J. Jeon and Y. Jee, “The JNK/NFκB pathway is required to activate murine lymphocytes induced by a sulfated polysaccharide from Ecklonia cava,†Biochimica et Biophysica Acta (BBA)-General Subjects. Vol. 1830(3), pp. 2820-2829, 2013.

J.N. Liu, Y. Yoshida, M.Q. Wang, Y. Okai and U. Yamashita, “B cell stimulating activity of seaweed extracts,†International Journal of Immunopharmacology, vol. 19(3), pp. 135-142, 1997.

B.E. Shan, Y. Yoshida, E. Kuroda and U. Yamashita, “Brief Communication Immunomodulating activity of seaweed extract on human lymphocytes in vitro,†International Journal of Immunopharmacology, vol. 21(1), pp. 59-70, 1999.

S. Subaryono, R. Perangiangin, M.T. Suhartono and F.R. Zakaria, “Imunomodulator activity of alginate oligosaccharides from alginate Sargassum crassifolium,†Indonesian Fischeries Processing Journal, vol. 20(1), pp. 63-73, 2017.

S. Cox, N. Abu-Ghannam and S. Gupta, “Effect of processing conditions on phytochemical constituents of edible irish seaweed Himanthalia elongate,†Journal Food Processing Preservation, vol. 36(4), pp. 348-363, 2011.

B. Cervantes-Paz, E.M. Yahia, J. De Jesus Ornelas-Paz, C.I. Victoria-Campos, V. Ibarra-Junquera, Péeez-Martínez JD. et al., “Antioxidant activity and content of chlorophylls and carotenoids in raw and heat-processed Jalapeno peppers at intermediate stages of ripening,†Food Chemistry, vol. 146, pp. 188–196, 2014.

Erniati, F.R. Zakaria and B.P. Priosoeryanto, “The effect of fat free cocoa (Theobroma cacao L.) powder drinks consumption on antioxidative activity of lymphocyte of women subject,†Journal Food, Technology and Industry, vol. 23(1), pp. 81-85, 2012.

G. Ahn, E. Park, W.W. Lee, J.W. Hyun, K.W. Lee, T. Shin, Y.J. Jeon and Y. Jee, “Enzymatic extract from Ecklonia cava induces the activation of lymphocytes by IL-2 production through the classical NF-κB pathway,†Marine Biotechnology, vol. 13(1), pp. 66-73, 2013.

M. Colic and M. Savic, “Garlic extracts stimulate proliferation of rat lymphocytes in vitro by increasing il-2 and il-4 production,†Immunopharmacology And Immunotoxicology, vol. 22(I), pp. 163-181, 2000.

S. Sharma, N.P. Kalia, P. Suden, P.S. Chauhan, M. Kumar, A.B. Ram, A. Khajuria, S. Sarang Bani and I.A. Khan, “Protective efficacy of piperine against Mycobacterium tuberculosis,†Tuberculosis, vol. Xxx, pp. 1-8, 2014.

D.F.A. Elmegeed, D.A. Ghareeb, M. Elsayed and M. El-Saadani, “Phytochemical constituents and bioscreening activities of green algae Ulva lactuca,†International Journal Agricultural Policy Research, vol. 2(11), pp. 373-378, 2015.

L. Machu, L. Misurcova, J.V. Ambrozova, J. Orsavova, J. Mlcek, J. Sochor and T. Jurikova, “Phenolic content and antioxidant capacity in algal food products,†Molecules, vol. 20(1), pp. 1118-1133, 2015.

H.A. El-Baky, F.K. El Baz and G.S. El-Baroty, “Evaluation of marine alga Ulva lactuca.as a source of natural preservative ingredient,†American-Eurasian. EJEAFChe, vol. 7(11), pp. 3353-3367, 2008.

S. Sharmila and R.J. Rebecca, “Phytochemical analysis of Enteromorpha flexuosa and Ulva lactuca: a comparative study,†International Journal Pharmaceutical and Biologycal Sciences, vol. 5(4), pp. 830 – 834, 2014.

F.R. Zakaria, Nurrahman, E. Prangdimurti and Tejasari, “Antioxidant and immunoenhancement activities of ginger (Zingiber officinale Roscoe) extracts and compounds in vitro and in vivo mouse and human system,†Nutraceuticals and Food, vol. 8(1), pp. 96-104, 2003.

R.F. Hasrini, F.R Zakaria, D.R. Adawiyah and I.H. Suparto, “Antidiabetic and immunomodulatory potential of purple soymilk enriched with crude palm oil microcapsule in type-2 diabetes mellitus respondents,â€Malaysian Journal of Nutrition, vol. 23(3), pp.461-471, 2017.

O.I. Hidenory, O. Takeshi, K, Teruno and K. Kazumori, “Activation of lymphocyte proliferation by boronate-containing polymer immobilized on substrate: The effect of boron content on lymphocyte proliferation,†European Cells and Materials,†vol. 12, pp. 36-43, 2006.

I.M. Roitt and P. J. Delves, “Essential Immunology,†10th edition. Blackwell Science Ltd. London, pp. 17-25, 75-81, 2001.

S.Y. Lyu and W.B. Park, “Production of cytokine and NO by RAW 264.7 macrophages and PBMC in vitro incubation with flavonoids,†Archives Pharmacology Research, vol. 28(5), pp. 573–581, 2005.

D. Ranjan, C. Chen, T. Johnston, H. Jeon and M. Nagabhushan, “Curcumin inhibits mitogenstimulated lymphocyte proliferation, NFκB activation, and IL-2 signaling,†Journal of Surgical Research, vol. 121(2), pp. 171–177, 2004.

F.R, Zakaria, L. Darsana and H. Wijaya, “Immunity enhancement and cell protection activity of ginger bud and fresh on mouse spleen lymphocyte,†Symposium Non-Nutritive Health Factors for Future Food. Corean Society of Food Science and Technology, September 28 – 30, 1996.

N. Rajapakse, S.K. Kim, “Nutritional and digestive health benefits of seaweed,†Adv in Food and Nutr Res, vol. 64, pp. 17-28, 2011.

B. Schechter, “Lymphocyte stimulation. In: Differential Sensitivy To Radiation Biochemical and Immunological Process,†Editor: Castellani A. New York (USA), Plenum Press, 2010.

I. Schepetkin, L. Kirpotina, L. Jakiw, A. Khlebnikov,C. Blaskovich, M. Jutila and M. Quinn, “Immunomodulatory activity of oenothein B isolated from Epilobium angustifolium,†Journal Immunology, vol. 183(10), pp. 6754–6766, 2009.




DOI: http://dx.doi.org/10.18517/ijaseit.10.3.9440

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