Antagonistic Fungal Endophytes Colonizing Rhizome of Amomum centrocephalum A.D. Poulsen from North Sumatera, Indonesia

Anisa Lutfia, Erman Munir, - Yurnaliza, Adrian Hartanto

Abstract


Endophytic fungi were successfully isolated from healthy rhizomes of the wild ginger Amomum centrocephalum A.D. Poulsen from Hutan Sibayak, North Sumatera, Indonesia. This study aimed to evaluate the antagonistic properties of isolated endophytic fungi against selected pathogenic bacteria and phytopathogenic fungi assessed by dual culture plate assay. Methanolic extract of A. centrocephalum rhizome was also tested as a control. Nine morphotypes of endophytic fungi were differentiated morphologically with further molecular identification using ITS rDNA sequencing for potential antagonistic strains. Most isolates showed considerable inhibitory activities towards microbial indicators than MeOH extract of A. centrocephalum. All strains showed distinct inhibitory activities against bacterial pathogens. The strongest activity was shown against gram-positive, Staphylococcus aureus ATCC 29213™ followed by gram-negative, Escherichia coli ATCC 25922™ while there was no evidence of inhibition against clinical yeast strain, Candida albicans. All strains were known as prominent antagonists of phytopathogens against Fusarium oxysporum, Ganoderma boninense, and Rigidoporus ligneous. The five potential strains were identified as Aspergillus aculeatus, Clonostachys rosea, Daldinia caldariorum, and two strains of Trichoderma. Hence, we reported the newly found species of endophytic fungi colonizing rhizomes of Sumatran Zingiberacean species with prospects upon finding novel metabolite compounds combating bacterial infection and plant diseases.

Keywords


Amomum centrocephalu; antagonism assay; endophytic fungi; medicinal plants; zingiberaceae.

Full Text:

PDF

References


R. H. Patil, M. P. Patil, and V. L. Maheshwari, “Bioactive Secondary Metabolites From Endophytic Fungi: A Review of Biotechnological Production and Their Potential Applications,” Stud. Nat. Prod. Chem., vol. 49, pp. 189–205, 2016.

S. M. Nalawade and H.-S. Tsay, “In vitro propagation of some important chinese medicinal plants and their sustainable usage,” Vitr. Cell. Dev. Biol. - Plant, vol. 40, no. 2, pp. 143–154, 2004.

P. Vuorela et al., “Natural products in the process of finding new drug candidates.,” Curr. Med. Chem., vol. 11, no. 11, pp. 1375–1389, 2004.

G. A. Strobel, “Endophytes as sources of bioactive products,” Microbes Infect., vol. 5, no. 6, pp. 535–544, 2003.

G. Strobel and B. Daisy, “Bioprospecting for Microbial Endophytes and Their Natural Products,” Microbiol. Mol. Biol. Rev., vol. 67, no. 4, pp. 491–502, 2003.

D. J. Newman and G. M. Cragg, “Natural products as sources of new drugs over the last 25 years,” J. Nat. Prod., vol. 70, no. 3, pp. 461–477, 2007.

J. Hallmann, G. Berg, and B. Schulz, “Isolation Procedures for Endophytic Microorganisms,” Microb. Root Endophytes, vol. 9, pp. 299–319, 2006.

K. H. Kogel, P. Franken, and R. Hückelhoven, “Endophyte or parasite - what decides?,” Curr. Opin. Plant Biol., vol. 9, no. 4, pp. 358–363, 2006.

K. Saikkonen, S. Saari, and M. Helander, “Defensive mutualism between plants and endophytic fungi?,” Fungal Divers., vol. 41, no. January, pp. 101–113, 2010.

X. Sun and L. D. Guo, “Endophytic fungal diversity: Review of traditional and molecular techniques,” Mycology, vol. 3, no. 1, pp. 65–76, 2012.

F. dan S. N. Hartanto, S., “Studi Etnobotani Famili Zingiberaceae dalam Kehidupan Masyarakat Lokal di Kecamatan Pangean Kabupaten Kuantan Singingi, Riau,” Biosaintifika, vol. 6, no. 2, pp. 98–108, 2014.

A. Auliani, Fitmawati, and N. Sofiyanti, “Studi ethnobotany famili zingiberaceae dalam kehidupan masyarakat lokal di kecamatan siak hulu kabupaten kampar,” Jom Fmipa, vol. 1, no. 2, pp. 526–533, 2014.

M. S. Nahdi, N. A. Martiwi, and D. C. Arsyah, “The ethnobotany of medicinal plants in supporting the family health in Turgo, Yogyakarta, Indonesia,” BIODIVERSITAS, vol. 17, no. 2, pp. 900–906, 2016.

R. Kumar, O. Prakash, A. K. Pant, V. A. Isidorov, and C. S. Mathela, “Chemical composition, antioxidant and myorelaxant activity of essential oils of Globba sessiliflora Sims,” J. Essent. Oil Res., vol. 24, no. 4, pp. 385–391, 2012.

B. Sabulal, V. George, M. Dan, and N. S. Pradeep, “Chemical Composition and Antimicrobial Activities of the Essential Oils from the Rhizomes of Four Hedychium Species from South India,” J. Essent. Oil Res., vol. 19, no. 1, pp. 93–97, 2007.

E. W. C. Chan and S. K. Wong, “Phytochemistry and pharmacology of ornamental gingers, Hedychium coronarium and Alpinia purpurata: a review,” J. Integr. Med., vol. 13, no. 6, pp. 368–379, 2015.

J. C. Ho, “Antimicrobial, mosquito larvicidal and antioxidant properties of the leaf and rhizome of Hedychium coronarium,” J. Chinese Chem. Soc., vol. 58, no. 4, pp. 563–567, 2011.

B. Bussaban, S. Lumyong, P. Lumyong, E. H. C. McKenzie, and K. D. Hyde, “Endophytic fungi from Amomum siamense,” Can. J. Microbiol., vol. 47, no. 10, pp. 943–948, 2001.

Yurnaliza, I. N. P. Aryantha, R. R. Esyanti, and A. Susanto, “Antagonistic activity assessment of fungal endophytes from Oil palm tissues against Ganoderma boninense Pat,” Plant Pathol. J., vol. 13, no. 4, pp. 257–267, 2014.

M. Balouiri, M. Sadiki, and S. K. Ibnsouda, “Methods for in vitro evaluating antimicrobial activity: A review,” J. Pharm. Anal., vol. 6, no. 2, pp. 71–79, Apr. 2016.

M. R. Bivi, M. S. N. Farhana, A. Khairulmazmi, and A. Idris, “Control of Ganoderma boninense : A Causal Agent of Basal Stem Rot Disease in Oil Palm with Endophyte Bacteria In Vitro Control of Ganoderma boninense : A Causal Agent of Basal Stem Rot Disease in Oil Palm with Endophyte Bacteria In Vitro,” Int. Journa Agric. Biol., vol. 12, no. November 2009, pp. 12:833-839, 2010.

D. K. Manter and J. M. Vivanco, “Use of the ITS primers, ITS1F and ITS4, to characterize fungal abundance and diversity in mixed-template samples by qPCR and length heterogeneity analysis,” J. Microbiol. Methods, vol. 71, no. 1, pp. 7–14, 2007.

R. C. Edgar, “MUSCLE: Multiple sequence alignment with high accuracy and high throughput,” Nucleic Acids Res., vol. 32, no. 5, pp. 1792–1797, 2004.

K. Tamura, G. Stecher, D. Peterson, A. Filipski, and S. Kumar, “MEGA6: Molecular evolutionary genetics analysis version 6.0,” Mol. Biol. Evol., vol. 30, no. 12, pp. 2725–2729, 2013.

N. Saitou and M. Nei, “The neighbor-joining method: a new method for reconstructing phylogenetic trees.,” Mol. Biol. Evol., vol. 4, no. 4, pp. 406–425, 1987.

J. Felsenstein, “Confidence Limits on Phylogenies : An Approach Using the Bootstrap,” Society, vol. 39, no. 4, pp. 783–791, 1985.

E. S. Siregar and N. Pasaribu, “Inventarisasi Jenis-Jenis Zingiberaceae Di Hutan Sibayak Sumatera Utara,” J. Penelit. MIPA, vol. 2, no. 1, pp. 22–24, 2008.

M. Habsah et al., “Screening of Zingiberaceae extracts for antimicrobial and antioxidant activities,” J. Ethnopharmacol., vol. 72, no. 3, pp. 403–410, 2000.

R. Kurup, V. P. Thomas, J. Jose, M. Dan, M. Sabu, and S. Baby, “Chemical Composition of Rhizome Essential Oils of Amomum agastyamalayanum and Amomum newmanii from South India,” J. Essent. Oil Bear. Plants, vol. 21, no. 3, pp. 803–810, 2018.

A. K. Hazarika and S. C. Nath, “Methyl chavicol—the major component of the rhizome oil of Amomum linguiforme Benth.,” J. Essent. Oil Res., vol. 7, no. 3, pp. 325–326, 1995.

B. Sabulal, V. George, and M. Dan, “Composition of rhizome and leaf oils of amomum pterocarpum thwaites,” J. Essent. Oil Res., vol. 19, no. 1–4, pp. 23–25, 2007.

R. C. B. Ginting, N. Sukarno, U. Widyastuti, L. K. Darusman, and S. K. Kanaya, “Diversity of Endophytic Fungi from Red Ginger (Zingiber officinale Rosc.) Plant and Their Inhibitory Effect to Fusarium oxysporum Plant Pathogenic Fungi,” HAYATI J. Biosci., vol. 20, no. 3, pp. 127–137, 2013.

H. Winarsi and S. S. Susilowati, “Functional-drink rich in antioxidant cardamom-rhizome (Amomum cardamomum willd) suppresses inflammation and improves lipid profile,” IOP Conf. Ser. Earth Environ. Sci., vol. 102, no. 1, 2018.

C. Anisha and E. K. Radhakrishnan, “Metabolite analysis of endophytic fungi from cultivars of Zingiber officinale Rosc. identifies myriad of bioactive compounds including tyrosol,” 3 Biotech, vol. 7, no. 2, pp. 1–10, 2017.

F. Uzma, N. M. Konappa, and S. Chowdappa, “Diversity and extracellular enzyme activities of fungal endophytes isolated from medicinal plants of Western Ghats, Karnataka,” Egypt. J. Basic Appl. Sci., vol. 3, no. 4, pp. 335–342, 2016.

S. K. Deshmukh, S. A. Verekar, and S. V. Bhave, “Endophytic fungi: A reservoir of antibacterials,” Front. Microbiol., vol. 5, pp. 1–43, 2015.

U. S. Hastuti, D. Rahmawati, R. Y. Sari, R. D. Fitri, and P. M. Al Asna, “Antimicrobial activity of endophytic fungi isolated from a medicinal plant, Hedychium acuminatum Roscoe,” in AIP Conference Proceedings, 2018, vol. 050002, no. 2018, p. 050002.

A. Pansanit and P. Pripdeevech, “Antibacterial secondary metabolites from an endophytic fungus, Arthrinium sp. MFLUCC16-1053 isolated from Zingiber cassumunar,” Mycology, vol. 00, no. 00, pp. 1–9, 2018.

D. M. Hillis and J. J. Bull, “An Empirical Test of Bootstrapping as a Method for Assessing Confidence in Phylogenetic Analysis,” Syst. Biol., vol. 42, no. 2, pp. 182–192, 1993.

V. Narsian and H. H. Patel, “Aspergillus aculeatus as a rock phosphate solubilizer,” Soil Biol. Biochem., vol. 32, no. 4, pp. 559–565, 2000.

J. C. Sutton, W. Liu, J. Ma, W. G. Brown, J. F. Stewart, and G. D. Walker, “Evaluation of the fungal endophyte Clonostachys rosea as an inoculant to enhance growth, fitness and productivity of crop plants,” Acta Hortic., vol. 782, pp. 279–286, 2008.

M. E. Da Silva et al., “Efficacy of Clonostachys rosea and Duddingtonia flagrans in Reducing the Haemonchus contortus Infective Larvae,” Biomed Res. Int., vol. 2015, pp. 10–14, 2015.

R. Roberti, F. Badiali, A. Pisi, A. Veronesi, D. Pancaldi, and A. Cesari, “Sensitivity of Clonostachys rosea and Trichoderma spp. as potential biocontrol agents to pesticides,” J. Phytopathol., vol. 154, no. 2, pp. 100–109, 2006.

S. Mukesh, K. Vipul, S. Mohammad, P. Sonika, ey, and S. Anuradha, “Trichoderma- a potential and effective bio fungicide and alternative source against notable phytopathogens: A review,” African J. Agric. Res., vol. 11, no. 5, pp. 310–316, 2016.




DOI: http://dx.doi.org/10.18517/ijaseit.9.4.8781

Refbacks




Published by INSIGHT - Indonesian Society for Knowledge and Human Development