Agarwood Leaf Essential Oil Characterization and Effects on MCF-7 Breast Cancer Cells

Nur A. A. Zainurin, Yumi Z. H Y. Hashim, Nor F. Mohamed Azmin, Phirdaous Abbas

Abstract


Breast cancer continues to remain as the leading cause of cancer mortality among women worldwide. Agents for prevention and cure for breast cancer are contiuously being researched. Ä°n particular, agarwood essential oil from resin infiltrated heartwood has been reported to have substantial evidences of medicinal benefits. Nevertheless, there is very limited report on agarwood leaf essential oil (ALEO). Hence, this present study was conducted to evaluate the physicochemical properties, chemical constituents and anticancer activity of ALEO. ALEO was observed to be of pale-yellow colour with sweet smell. Other physicochemical properties include refractive index of 1.44, specific gravity of 0.886, saponification value of 131.88 mg KOH/g, acid value of 2.80 mg KOH/g and iodine value of 105.07 gI2/100g. The profiling of chemical constituents using gas chromatography-mass spectrometry (GCMS) revealed 19 compounds. Hexadecanoic acid was the major compound (64.41%). The biomarkers of agarwood; azulene (0.619%) and guaiol (0.2997%) were also detected. ALEO was tested for anticancer activity against MCF-7 cancer cells using WST-8 assay. ALEO showed the IC50 value of 31% (v/v) against MCF-7 cells after 36 hours of treatment. In conclusion, this study provides information on ALEO physicochemical properties and chemical constituents that can be used as benchmark for quality assurance as well as proof that ALEO holds a potential as anticancer agent.


Keywords


Agarwood leaf; breast cancer; essential oil; GCMS; MCF-7.

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References


L. A. Torre, F. Bray, R. L. Siegel, J. Ferlay, J. Lortetâ€Tieulent and A. Jemal, “Global cancer statistics, 2012,†CA: a cancer journal for clinicians, vol. 65(2), pp. 87-108, Mar. 2015.

J. Iqbal, B.A. Abbasi, T. Mahmood, S. Kanwal, B. Ali and A. T. Khalil, “Plant-derived anticancer agents: A green anticancer approach,†Asian Pacific Journal of Tropical Biomedicine, Nov. 2017.

(2018) WHO, World Health Organization website. [Online]. Available: http://www.who.int/en/news-room/fact-sheets/detail/cancer

(2018) Cancer Research UK website. [Online]. Available: https://www.cancerresearchuk.org/about-cancer/breast-cancer/about

K. J. Chavez, S. V. Garimella and S. Lipkowitz, “Triple negative breast cancer cell lines: one tool in the search for better treatment of triple negative breast cancer,†Breast disease, vol. 32(1-2), p. 35, 2010.

K. L. Maughan, M. A. Lutterbie and P. S. Ham, “Treatment of breast cancer,†Chemotherapy, vol. 51, p. 53, 2010.

S. Akter, M. T. Islam, M. Zulkefeli and S. I. Khan, “Agarwood production-a multidisciplinary field to be explored in Bangladesh,†International Journal of Pharmaceutical and Life Sciences, vol. 2(1), pp. 22-32, 2013.

H. H. Muhammad, M. F. Azlina, C. M. Hasfalina, Z. A. Zurina and J. Hishamuddin, “Optimization and kinetic study of gaharu oil extraction,†World Academy of Science, Engineering and Technology, International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering, vol. 7(6), pp. 454-7, Jun 2013.

Y. Z. H-Y. Hashim, P. G. Kerr, P. Abbas and H. M. Salleh, â€Aquilaria spp. (agarwood) as source of health beneficial compounds: A review of traditional use, phytochemistry and pharmacology,†Journal of Ethnopharmacology, vol. 189, pp. 331-60, Aug. 2016.

Y. Z. H-Y. Hashim, A. Phirdaous and A. Azura, “Screening of anticancer activity from agarwood essential oil,†Pharmacognosy research, vol. 6(3), p. 191, Jul. 2014.

S.S.Dahham, Y. M. Tabana, M. A. Iqbal, M. B. Ahamed, M. O. Ezzat, A. S. Majid and A. M. Majid, “The anticancer, antioxidant and antimicrobial properties of the sesquiterpene β-caryophyllene from the essential oil of Aquilaria crassna,â€. Molecules, vol. 20(7), pp. 11808-29, Jun 2015.

S.S. Dahham, Y. M. Tabana, L. E. Hassan, M. B. Ahamed, A.S. Majid and A. M. Majid, “In vitro antimetastatic activity of Agarwood (Aquilaria crassna) essential oils against pancreatic cancer cells,†Alexandria Journal of Medicine, vol. 52(2), pp. 141-50, 2016.

A. B. Caburian and M. O. Osi, “Characterization and evaluation of antimicrobial activity of the essential oil from the leaves of Piper betle L.,†E-International Scientific Research Journal, vol. 2(1), pp. 2-13, 2010.

(2018) Sucrose Conversion Table. [Online]. Available: https://www.greenwoodassociates.com/certs/Detailed%20Brix%20Chart.pdf.

A. Author, “International commission for uniform methods of sugar analysis. Report of the proceedings of the ninth session, 1936,†The Analyst, vol. 62(732), pp. 197-200, 1937.

M. P. Paudyal, M. Rajbhandari, P. Basnet, S. Yahara and M. B. Gewali, “Quality assessment of the essential oils from Nardostachys jatamansi (D. Don) DC and Nardostachys chinensis batal obtained from Kathmandu valley market,†Scientific World, vol. 10(10), pp. 13-6, Jul. 2012.

I. M. Barkatullah, A. Rauf and K. Inyat-Ur-Rahman,†Physicochemical characterization of essential and fixed oils of Skimmia laureola and Zanthoxylum armatum,†Middle-East J. Med. Plants Res, vol. 1, pp. 51-8, 2012.

AOAC (Association of Official Analytical Chemists), Official methods of analysis. Gaithersburg, MD, Washington, USA, 2000.

Y. Z. Hashim, N. I. Ismail and P. Abbas, “Analysis of chemical compounds of agarwood oil from different species by gas chromatography mass spectrometry (GCMS),†IIUM Engineering Journal, vol. 15, p. 31, May, 2014.

H. Tominaga, M. Ishiyama, F. Ohseto, K. Sasamoto, T. Hamamoto, K. Suzuki and M. Watanabe, “A water-soluble tetrazolium salt useful for colorimetric cell viability assay,†Analytical Communications, vol. 36(2), pp. 47-50, 1999.

S. Sahidin, S. Nakazibwe, M. Taher and A. K. Saxena, “SJA Ichwan Antiproliferative activity of Curcusone B from Jatropha curcas on human cancer cell lines,†Aust. J. Basic Appl. Sci. Vol. 5, pp. 47-51, 2011.

M. N. Boukhatem, F. M. Amine, A. Kameli, F. Saidi, K. Walid and S. B. Mohamed, “Quality assessment of the essential oil from Eucalyptus globulus Labill of Blida (Algeria) origin,†International Letters of Chemistry, Physics and Astronomy, vol. 17(3), pp. 303-15, 2014.

S. Clarke. Essential chemistry for aromatherapy. Elsevier Health Sciences, 2008.

H. Rahman, K. Vakati and M. C. Eswaraiah, “In-vivo and in-vitro anti-inflammatory activity of Aquilaria agallocha oil,†International Journal of Basic Medical Sciences and Pharmacy, vol. 2(1), Jun 2012.

D. Mishra, “Preparation of soap using different types of oils and exploring its properties†PhD. thesis, Department of Chemical Enineering. National Institute of Technology, Rourkela, India, 2013.

C. Alimentarius, Codex alimentarius standards for fats and oils from vegetable sources. Codex alimentarius standards for named vegetable oil. Codex Alimentarius Stan., vol. 210, 1999

S. Dileesh, M. Adithya, A. Sankar and C. V. Peter, “Determination of saponification, acid and ester values; percentage of free fatty acids and glycerol in some selected edible oils: Calculation of concentration of lye needed to prepare soap from these oils,†Research Scholar, pp. 2249-6696, 2013.

C. Turek and F. C. Stintzing, “Stability of essential oils: a review,†Comprehensive Reviews in Food Science and Food Safety. vol. 12(1), pp. 40-53, Jan. 2013.

D. Tiran, Aromatherapy in Midwifery Practice. Singing Dragon, Jul. 2016.

H. Panda, Aromatic Plants Cultivation, Processing and Uses. Asia Pacific Business Press Inc., 2005.

M. Kilonzo, P. A. Ndakidemi and M. Chacha, “Mystroxylon aethiopicum chloroform root bark extracts phytochemical analysis using gas chromatography mass spectrometry,†Journal of Pharmacognosy and Phytotherapy, vol. 9(4), pp. 44-50, Apr. 2017.

L. Yufeng, Y. Xiuwei and L. Tonghua, “GC-MS Analysis of essential oil from the leaves of Aquilaria sinensis [J],â€Modern Chinese Medicine vol. 8, p. 4, 2007.

H. Chen, Y. Yang, J. Xue, J. Wei, Z. Zhang and H. Chen, “Comparison of compositions and antimicrobial activities of essential oils from chemically stimulated agarwood, wild agarwood and healthy Aquilaria sinensis (Lour.) Gilg trees,†Molecules, vol. 16(6), pp. 4884-96, Jun. 2011.

M. N. I. Bhuiyan, J. Begum and M. N. H. Bhuiyan, “Analysis of essential oil of eaglewood tree (Aquilaria agallocha Roxb.) by gas chromatography mass spectrometry,†Bangladesh J Pharmacol., vol. 4, pp. 24-8, Jan. 2009.

M. R. Serafini, D. M. Vergne, T. K. Rabelo, P. Menezes, R. Rocha, J. C. Moreira, F. Silva, P. B. Alves, H. Jesus, A. A. Araújo and D. P. Gelain, “Determination of chemical and physical properties of Hyptis pectinata essential oil and their redox active profile,†Journal of Biotechnology and Pharmaceutical Research, vol. 3(1), pp. 1-9, 2012.




DOI: http://dx.doi.org/10.18517/ijaseit.8.4-2.7034

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