Saccharification Waste Biomass Rice Straw IR-64 by Using Xylanase from Indigenous Marine Bacteria Bacillus safensis LBF-002

Abstract

Abstract— Agricultural residues have an enormous potential as renewable carbon and energy sources. Saccharification of agricultural by microbial hydrolytic enzymes is the first step of bioconversion of organic material into reducing sugar. The main purpose of this research is converting rice straw IR-64 waste biomass into reducing sugar xylo-oligosaccharides by using xylanase from indigenous marine bacteria Bacillus safensis LBF-002. The optimation of xylanase in rice straw medium are using stability of pH and temperature were resulted higher activity in pH 7 and 50 °C which result 2.228 U/mL in 24 h incubation. The xylanase was concentrated with PEG 6000 in ratio (1:1) become 16.578 U/mL and was used for hydrolyze the rice straw for getting the sugar reduction product. The sugar reduction component from rice straw saccharification was analyzed using Thin Layer Chromatography and also High Performance Liquid Chromatography (HPLC). The present study was a first effort to explore marine bacteria Bacillus safensis LBF-002 to produce and obtain the optimum condition for producing xylooligosaccharide from rice straw waste. The best result for hydrolysis experiment showed in saccharafication with 2.5% rice straw and crude enzyme xylanase 4 U/mL for 1 h and 2 h incubation which is resulted xylose, xylobiose, and xylohexose.