Morphological and Molecular Identification of Ramie Plants (Boehmeria nivea L. Gaud) in Indonesia

Reni Mayerni, Sari Rukmana Okta Sagita Chan, - Gustian

Abstract


This research has been conducted at the Experimental Farm of Agricultural Faculty of Andalas University and the Laboratory of the Department of Agronomy and Horticulture Agricultural Faculty, IPB University, Bogor. The purpose of this study was to determine the genetic diversity with similarity analysis of the ramie plant collection of Experimental Farm, Agricultural Faculty of Andalas University. This research was conducted employing morphological and molecular characterization. Morphological characterization of the ramie plant is done by observing the character 17 qualitative and 15 quantitative characters ramie plant. While the molecular characterization using RAPD markers (Random Amplified Polymorphic DNA) with ten primers. The results of morphological characters, which serve as distinguishing characteristics of the five clones, is the color of the leaf. The results of the qualitative character of phenotypic variance analysis of variability obtained plants with broad criteria contained in the 4-character observation (the color of the leaf petiole, the leaf color, flower color, and female sex flowers). Similarity analysis based on morphological characters is qualitatively classified into two groups with the degree of similarity coefficient from 0.34 to 0.75. Similarity analysis of qualitative and quantitative combined data grouped into two groups with the degree of similarity coefficient from 0.02 to 0.20. The similarity analysis based on molecular characters is grouped into two groups with a similarity coefficient level of 0.27 to 0.75.


Keywords


ramie plants; genetic Diversity; characterization; morphology; RAPD.

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References


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DOI: http://dx.doi.org/10.18517/ijaseit.10.3.5258

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