Isolation and Characterization of Scopoletin from The Bark of fagraea ceilanica thumb and Antioxidants Tests

Abstract

Have done the isolation scopoletin compound from the bark of fagraea ceilanica Thunb by maceration method using n-hexane, ethyl acetate and methanol. Column chromatography with silica gel used for the isolation of the ethyl acetate extract fraction with a mixture of n-hexane and ethyl acetate as the mobile phase in the SGP (step Gradient Polarity). This way produces a dark yellow crystals (26 mg) with a melting point = 205-207 ° C. TLC (Thin Layer Chromatography) were eluted using a mixture of n-hexane: ethyl acetate (7: 3) gives (Rf 0.42). Based on the UV spectrum, shift reagents, IR spectrum and melting point of this compound compared with standard scopoletin showed that the isolated compounds are the same compound or isolated compound is scopoletin. The results of antioxidant test with DPPH of isolated compounds as weak antioxidants  with IC 50 = 358.71 mg / L.