Optimization of Production Xylanase from Marine Bacterium Bacillus safensis P20 on Sugarcane Baggase by Submerged Fermentation

Abstract

Endo-1, 4-β-xylanase commonly called xylanase is an industrially important enzyme which degrades of lignocellulosic materials to sugar, alcohol and other useful product. The use of commercially xylan is too expensive for use at industrial scale production. For commercial applications, xylanases should ideally be produced from simple and inexpensive substrates. Indonesia has abundantly agro-residues such as sugar cane bagasse which is attractive to be used as carbon sources for the production of enzyme.  In this study, optimization of fermentation condition extracellular xylanasefrom marine bacterium, Bacillus safensisP20 has been conducted by using sugarcane bagasse as carbon source under sub merged fermentation (SMF). Maximum xylanase production was obtained at sugar cane bagasse concentration 1.5%, pH medium 7, and temperature fermentation 20^oC, lactose as a carbone source and urea as a nitrogen source with activity 4.06 U/mL for 96 hours.