Induction of Hairy roots of Pegagan (Centella Asiatica (l.) Urban using Several Explant Sources with Several Agrobacterium Rhizogenes Strains in Vitro

Abstract

Induction of hairy roots of pegagan using A. rhizogenes strains is important to obtain high production of triterpenoid as secondary metabolite.  The objectives of this research were to obtain the best A. rhizogenes strains and the best explant sources for C. asiatica hairy root induction. The research consisted of two stages. Stage I was A. rhizogenes arranged in  Completely Randomized Design  (CRD) with 3 treatments and 6 replications. As a treatment was inoculation with A. rhizogenes strains LBA 9457, A4,  R1000 and control.  Stage II  was explant sources trial arranged in CRD with 3 treatments and 6 replications. The treatments were: internodus, apical bud and leaves. The results showed that all three strains of A. rhizogenes were able to induce C. asiatica plant to produce hairy roots, with R1000 as the best strain.The best explant source was explant leave with  15 day after inoculation and the percentage of  C. asiatica  hairyroot formation was 100 %. The β-glucoronidase (  GUS) test result has proven that Ri plasmid T-DNA of A. rhizogenes  was integrated into the genome of C. asiatica plant.