Construction of Hevea brasiliensis Genetic Linkage Map and Identification of Quantitative Trait Loci Using RAPD Markers

Novalina Novalina, Aidi Daslin Sagala

Abstract


Rubber breeding program so far have constraints especially in selection process which need very wide area and very long time. The use molecular markers in breeding program could simplify the selection procedure. The objectives of research were to get the linkage map especially for PB 260 as female parent, and to get RAPD markers and morphology markers which associated with QTL controlling production component. Construction of genetic linkage map on rubber tree (2n=36) was conducted by using RAPD markers data through pseudo-testcross. First generation progenies of crossing between PB 260 and PN were used as mapping populations: 22 progenies of A population (PB 260 x PN 7111), 20 progenies of C population (PB 260 x PN7). Construction of linkage map was conducted using MAPMAKER/EXP 3.0. Linkage map of PB 260 was constructed on LOD 3.0 yielded 1 linkage group, on LOD 2.5 yielded 2 linkage group. Based on single marker analysis through t-test was known that marker C13-2000 linked with yield character and number of latex vessel character. Character of leaf shape linked with girth, bark thickness, and number of latex vessel. Character of leaf edge linked with plugging index.

Keywords


Genetic Linkage Map; Rapd Markers; Qtl; First Generation Population of Rubber Tree; Double Pseudo-Testcross.

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Published by INSIGHT - Indonesian Society for Knowledge and Human Development