Photoacoustic for Oral Soft Tissue Imaging based on Intensity Modulated Continuous-Wave Diode Laser

Rini Widyaningrum, Dewi Agustina, Munakhir Mudjosemedi, Mitrayana -


We built the photoacoustic imaging (PAI) systems to image oral soft tissue. To reduce the cost and size significantly, the PAI system used an intensity-modulated continuous-wave (CW) diode laser with a wavelength of 532nm and output peak power of 200mW as an excitation source, combined with a condenser microphone as photoacoustic signals detector. The Pulse Width Modulation technique was applied to form a square wave fluctuation of laser radiation by using certain duty cycle on single-frequency of 17.8 kHz. Sample of this study was Sprague Dawley rats tongue on plasticine media, irradiated by modulated CW diode laser. The result of this study showed that modulated laser exposure on one certain spot of the sample produced PA-signals in polynomial correlation with a duty cycle of laser modulation. Based on it, the photoacoustic imaging then was done by using gradual duty cycles, i.e., 20%, 30%, and 40%. This study also showed that using CW diode laser which is modulated with low duty cycle can produce most accurate PA image, as well as keeping the sample from the high energy of laser exposure that may cause biological changes. Furthermore, the maximum duty cycle to modulate laser for oral soft tissue imaging in this system was 30%.


Photoacoustic imaging; diode laser; oral; soft tissue; modulated laser

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K. S. Valluru, B. K. Chinni, and N. A. Rao, “Photoacoustic Imaging : Opening New Frontiers in Medical Imaging,” J. Clin. Imaging Sci., vol. 1, no. 24, pp. 1–7, 2011.

R. R. Shantiningsih and S. F. Diba, “Efek Aplikasi Patch Gingiva Mukoadesif β-Carotene Akibat Paparan Radiografi Panoramik,” Maj Ked Gi Ind, vol. 1, no. 2, pp. 186–192, 2015.

J. Laufer, P. Johnson, E. Zhang, B. Treeby, B. Cox, B. Pedley, and P. Beard, “In vivo preclinical photoacoustic imaging of tumor vasculature development and therapy,” J. Biomed. Opt., vol. 17, no. 5, p. 056016.1-8, 2012.

M. Mudjosemedi, R. Widyaningrum, and R. S. Gracea, “Perbedaan Hasil Pengukuran Horizontal pada Tulang Mandibula dengan Radiograf Panoramik,” Maj Ked Gi Ind, vol. 1, no. 1, pp. 78–85, 2015.

J. Na’am, J. Harlan, S. Madenda, and E. P. Wibowo, “The Algorithm of Image Edge Detection on Panoramic Dental X-Ray Using Multiple Morphological Gradient (mMG) Method,” Int. J. Adv. Sci. Eng. Inf. Technol., vol. 6, no. 6, pp. 1012–1018, 2016.

J. Santony and J. Na’am, “Infiltrate Object Extraction in X-ray Image by using Math-Morphology Method and Feature Region Analysis,” Int. J. Adv. Sci. Eng. Inf. Technol., vol. 6, no. 2, pp. 239–244, 2016.

D. Wu, L. Huang, M. S. Jiang, and H. Jiang, “Contrast Agents for Photoacoustic and Thermoacoustic Imaging: A Review,” Int. J. Mol. Sci., vol. 15, pp. 23616–23639, 2014.

H. Fatakdawala, S. Poti, F. Zhou, Y. Sun, J. Bec, J. Liu, D. R. Yankelevich, S. P. Tinling, R. F. Gandour-Edwards, D. G. Farwell, and L. Marcu, “Multimodal in vivo imaging of oral cancer using fluorescence lifetime, photoacoustic and ultrasound techniques,” Biomed. Opt. Express, vol. 4, no. 9, pp. 1724–1741, 2013.

Y. Lao, D. Xing, S. Yang, and L. Xiang, “Noninvasive photoacoustic imaging of the developing vasculature during early tumor growth,” Phys.Med. Biol., vol. 53, pp. 4203–4212, 2008.

K. S. Valluru and J. K. Willmann, “Clinical photoacoustic imaging of cancer,” Ultrasonography, vol. 35, pp. 267–280, 2016.

S. Zackrisson, S. M. W. Y. van de Ven, and S. S. Gambhir, “Light In and Sound Out: Emerging Translational Strategies for Photoacoustic Imaging,” Cancer Res., vol. 74, no. 4, pp. 979–1004, 2014.

X. Wang, J. B. Fowlkes, J. M. Cannata, H. Changhong, and P. L. Carson, “Photoacoustic Imaging with A Commercial Ultrasound System and A Custom Probe,” Ultrasound Med. Biol., vol. 37, no. 3, pp. 484–492, 2011.

L. V. Wang and L. Gao, “Photoacoustic microscopy and computed tomography: from bench to bedside,” Annu Rev Biomed Eng, vol. 16, pp. 155–185, 2014.

S. Mallidi, G. P. Luke, and S. Emelianov, “Photoacoustic imaging in cancer detection, diagnosis, and treatment guidance,” Trends Biotechnol., vol. 29, no. 5, pp. 213–221, 2011.

L. V Wang and J. Yao, “A Practical Guide to Photoacoustic Tomography in the Life Sciences,” Nat Methods, vol. 13, no. 8, pp. 627–638, 2016.

L. V. Wang, “Multiscale photoacoustic microscopy and computed tomography,” Nat. Photonics, vol. 3, pp. 503–509, 2009.

C. Li, C. Kim, and L.V.Wang, “Photoacoustic Tomography and Ultrasound-Modulated Optical Tomography,” in Handbook of Biomedical Optics, D. A. Boas, C. Pitris, and N. Ramanujam, Eds. New York, US: CRC Press, 2011, pp. 419–442.

M. Mehrmohammadi, S. J. Yoon, D. Yeager, and S. Y. Emelianov, “Photoacoustic Imaging for Cancer Detection and Staging,” Curr Mol Imaging, vol. 2, no. 1, pp. 89–105, 2013.

K. Maslov and L. V Wang, “Photoacoustic imaging of biological tissue with intensity-modulated continuous-wave laser,” J. Biomed. Opt., vol. 13, no. 2, pp. 1–5, 2008.

J. Yao and L. V Wang, “Sensitivity of photoacoustic microscopy,” Photoacoustics, vol. 2, pp. 87–101, 2014.

J. Xia, J. Yao, and L. V Wang, “Photoacoustic Tomography: Principles and Advances,” Prog. Electromagn. Res., vol. 147, pp. 1–22, 2014.

D. V Bageshwar, A. S. Pawar, V. V Khanvilkar, and V. J. Kadam, “Photoacoustic Spectroscopy and Its Applications – A Tutorial Review,” Eurasian J. Anal. Chem., vol. 5, no. 2, pp. 187–203, 2010.

T. J. Allen and P. C. Beard, “High power visible light emitting diodes as pulsed excitation sources for biomedical photoacoustics,” Biomed. Opt. Express, vol. 7, no. 4, pp. 1260–1270, 2016.

T. Wang, S. Nandy, H. S. Salehi, P. D. Kumavor, and Q. Zhu, “A low-cost photoacoustic microscopy system with a laser diode excitation,” Biomed. Opt. Express, vol. 5, no. 9, pp. 20097–20102, 2014.

B. Soroushian and X. Yang, “Measuring non-radiative relaxation time of fluorophores with biomedical applications by photoacoustic effect,” Biomed. Opt. Express, vol. 2, no. 10, pp. 2749–2760, 2011.

R. G. M. Kolkman, W. Steenbergen, and T. G. van Leeuwen, “In vivo photoacoustic imaging of blood vessels with a pulsed laser diode,” Lasers Med Sci, vol. 21, pp. 134–139, 2006.

P. Beard, “Biomedical photoacoustic imaging,” Interface Focus, vol. 1, pp. 602–631, 2011.

R. A. Convissar, Principles and Practice of Laser Dentistry. St. Louis, Missouri: Mosby Elsevier, 2011.

P. Mohajerani, S. Kellnberger, and V. Ntziachristos, “Frequency domain optoacoustic tomography using amplitude and phase,” Photoacoustics, vol. 2, no. 3, pp. 111–118, 2014.



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