The Potentials of Isolated Hexadecanoic Acid of Hydroid Aglaophenia cupressina Lamoureoux as an Antifungal Compound on the Rotten Strawberries Fragaria x ananassa Dutch. and Mango Mangifera indica L

Eva Johannes, Amran Laga, Magdalena Litaay, Dirayah Rauf Husain, Nur Haedar, Mustika Tuwo, Julisda Hernianti Muru, Julianto Pasandin

Abstract


Overcoming microbial contaminants in fresh fruits is not merely by recognizing the level of contamination. Still, it requires another effort, such as applying a compound of natural and effective ingredients proven effective in reducing microbial contaminants and safe for health. The hexadecanoic acid used in this study was isolated from the tropical marine hydroids Aglaophenia cupressina Lamoureux. This study aimed to analyze the ability of bioactive compounds acid from the hydroid Aglaophenia cupressina Lamoureoux to inhibit the growth of fungi that cause rotten strawberry Fragaria x ananassa Dutch and mango Mangifera indica. Hexadecanoic acid was obtained by isolating it from the hydroid Aglaophenia cupressina Lamoureoux through the maceration, fractionation, and purification stages. Isolating fungi was done by using the PDA (Potato Dextrose Agar) medium to characterize macroscopically and microscopically and to test the inhibition using the diffusion method, which was incubated for 48 hours and 72 hours at the hexadecanoic acid concentrations of 15 ppm, 30 ppm, and 45 ppm. The results showed the hexadecanoic acid concentration of 45 ppm in the 72-hour incubation could inhibit the growth of two fungal isolates on strawberries, Fragaria x ananassa Dutch, i.e., Botrytis cinerea and Rhizopus stolonifer, for successive concentration, 24.00 mm and 22.75 mm. Meanwhile, the growth of Aspergillus niger, fungi from mangoes, could be inhibited by the hexadecanoic acid by 14.75 mm, 18.25 mm, and 23.50 mm, respectively, for the concentration of 15 ppm, 30 ppm, and 45 ppm with the 72-hour incubation.

Keywords


Aglaophenia cupressina Lamoureoux; acid, antifungal Fragaria x ananassa Dutch; antifungal Mangifera indica.

Full Text:

PDF

References


E. Johannes, E. Ishak, H. Usman, and M. Bilang, 'Effectiveness of Extracted Antibacterial Compound from Hydroid Aglaophenia cupressina Lamoureoux Against Bacterial Cell of Escherichia coli', International Journal of Scientific and Technology Research., vol. 2, no. 2, pp. 138-43, 2013.

E. Johannes, M. Litaay, and Syahribulan, 'The Bioactivity of Hexadecanoic Acid Compound Isolated from Hydroid Aglaophenia cupressina Lamoureoux as Antibacterial Agent Against Salmonella typhi', International Journal of Biological & Medical Research., vol. 7, no. 2, pp. 5469-5472, 2016.

N. S. Njerua, J. M. and Muema, 'Antimicrobial activity, phytochemical characterization and gas chromatography-mass spectrometry analysis of Aspilia pluriseta Schweinf. Extracts', J. Heliyon., vol 6, 2020.

S. Aurora, S. A. Silva, M. Carpena, P. Garcia-Oliveira, P. Gullón, M. F. Barroso, M. A. Prieto, J. Simal-Gandara, Review Macroalgae as a Source of Valuable Antimicrobial Compounds: Extraction and Applications, J. Antibiotics. 2020.

D.Chao, X. Meng, J. Meng, Md. Iqbal, H. Khan, L. Dai, A. Khan, X.An, J. Zhang, T. Huq, Y. Ni, 'Chitosan as A Preservative for Fruits and Vegetables: A Review on Chemistry and Antimicrobial Properties', Journal of Bioresources and Bioproducts., volume 4, pp 11-21 , 2019.

S. Aliasgarian, H. R. Ghassemzadeh, M. Moghaddam, and H. Ghaffari, 'Mechanical Damage Of Strawberry During Harvest And Postharvest Operations', Acta Technologica Agriculturae., vol. 1, pp. 1-5, 2015.

U. P. Lopes, L. Zambolim, D. B. Pinho, A. V. Barros, H. Costa, and O. L. Pereira, 'Postharvest Rot and Mummification of Strawberry Fruits Caused by Neofusicoccum parvum and N. kwambonambiense in Brazil', Journal Trop. Plant Pathol., vol. 39, no. 2, pp. 178-183, 2014.

E.Soesiladi, Widodo, Suskandini, R. Dirmawati, Z. Zulferiyenni, Rachmansyah A. Wardhana, F. Fitria, A. Fitri, J. Fajryah, ‘Effects of Fruit Coatings, Fungicide, and Storage Temperature on Fruit Shelf-Life and Qualities of ‘California’ Papaya’, J. Planta Tropika. vol 6, no. 1, 2018.

S.M. Yahaya, A.Y. Mardiyy, Review of Post-Harvest Losses of Fruits and Vegetables. Biomedical J. Of Scientific & Technical Research., vol. 10.2019.

S. Aliasgarian, H. R. Ghassemzadeh, M. Moghaddam, and H. Ghaffari, 'Mechanical Damage Of Strawberry During Harvest And Postharvest Operations', Acta Technologica Agriculturae., vol. 1, pp. 1-5, 2015.

S. Sadiq, M. S. Hashmi, I. Mabood, Q. Y. Durrani, A. Sarkhosh, I. Hussain, J. K.Brecht, 'Pre-storage chitosan-thyme oil coating control anthracnose in mango fruit', Scientia Horticulturae., vol. 284, 2021

Md. Mafizur Rahman, Md. Obyedul Kalam Azad, Jasim Uddain , Md. Adnan, Md. Chayan Ali, SK. Md. Jakaria Al-Mujahidy ,Md. Zohurul Kadir Roni, Mohammed Saifur Rahman ,Md. Jahirul Islam, Md. Hafizur Rahman , Ki Young Choi , Most Tahera Naznin, 'Microbial Quality Assessment and Efficacy of Low-Cost Disinfectants on Fresh Fruits and Vegetables Collected from Urban Areas of Dhaka, Bangladesh', Foods., vol. 10, pp. 1325. 2021

V.Tornero and G. Hanke, 'Chemical Contaminants Entering the Marine Environment From Sea-Based Sources: A Review with a Focus on European Seas', Marine Pollution Bulletin., vol. 112, pp. 17-38, 2016.

Adlhart C, J. Verran, N.F. Azevedo, H. Olmez , M.M. Keina¨nen-Toivola, I. Gouveia, L.F. Melo, F. Crijns. 'Surface modifications for antimicrobial effects in the healthcare setting: a critical overview', Journal of Hospital Infection., vol. 99, pp. 239-249. 2018.

D. Kevin Hyde, Jianchu Xu, 'The amazing potential of fungi: 50 ways we can exploit fungi industrially', Fungal Diversity., vol. 97, pp 1–136. 2019

K. Joanna, 'Peptides and protein hydrolysates as food preservatives and bioactive components of edible films and coatings-A review', Trends in Food Science & Technology., 106. 2020.

Md. N. Uddin, S. H. T. Shefat, M. Afroz, and N. J. Moon, 'Management of Anthracnose Disease of Mango Caused by Colletotrichum gloeosporioides: A Review', Acta Scientific Agriculture., vol. 10, no. 2, pp. 169-177, 2018.

N. Denis, H. Zhang, A. Leroux, R. Trudel, and H. Bietlot, 'Prevalence and Trends of Bacterial Contaminants and Ion in Fresh Fruit and Vegetables Sold at Retail in Canada', Journal Food Control., vol. 67, pp. 225-234, 2016.

P.Stefan , S. J. Knapp, J. A. L. van Kan, B. B. Ulate, 'Grey mould of strawberry, a devastating disease caused by the ubiquitous necrotrophic fungal pathogen Botrytis cinerea', Mol Plant Pathol., vol. 20, no. 6, pp. 877–892. 2019.

F. Jaumaux, L. P. G. de Cadiñanos, and P. Gabant, 'In the Age of Synthetic Biology, Will Antimicrobial Peptides be the Next Generation of Antibiotics?', Antibiotics., vol.9, no. 8, pp. 484, 2020.

A. Biharee, A. Sharma, A. Kumar, and V. Jaitak, 'Antimicrobial Flavonoids as a Potential Substitute for Overcoming Antimicrobial Resistance', Fitoterapia., vol. 146, pp. 104720, 2020.

X. Li, H. Zhao, and Xi. Chen, 'Screening of Marine Bioactive Antimicrobial Compounds for Plant Pathogens'. J. Marine Drugs., vol 19. No. 2, pp. 69, 2021.

K. Jung, M. Miyagawa, A. Matsuda, Y. Amagai, K. Oida, Y. Okamoto, M. Takai, S. Nishikawa, H. Jang, S. Ishizaka, G. Ahn, A. Tanaka, and H. Matsuda, 'Antifungal Effects of Palmitic Acid Salt and Ultrapure Soft Water on Scedosporium apiospermum', J Appl Microbiol., vol. 115, no. 3, pp. 711-7, 2013.

S. Mohammed, Al Aboody, 'Anti-Fungal Efficacy and Mechanisms of Flavonoids'. Journals Antibiotics., vol. 9 2020..

B. Somanon, S. Sae-Tia, B. C. Fries, 'Candidiasis and Mechanisms of Antifungal Resistance', J. Antibiotics. 2020

S. I. Anjuma, A. Ullaha, K. A. Khan, M. Attaullah, H. Khan, H. Ali, M. A. Bashir, M. Tahir, M. J. Ansari, H. A. Ghram, N. Adgabah, C. K. Dashk, 'Composition and functional properties of propolis (bee glue): A review', Saudi Journal of Biological Sciences., vol. 26, pp. 1695-1703, 2019.

K. Jürgen, R. Kolnaar, W. J. Ravensberg, 'Mode of Action of Microbial Biological Control Agents Against Plant Diseases: Relevance Beyond Efficacy', Front. Plant Sci., 2019

P. Balaji, G. Lianga, W. Liu, 'Immune defence to invasive fungal infections: A comprehensive review', Biomedicine & Pharmacotherapy., vol. 130, 2020.

G. R. Rubio, H. C. de Oliveira, J. Rivera, N. Trevijano-Contador, ‘The Fungal Cell Wall: Candida, Cryptococcus, and Aspergillus Species’, Front. Microbiol., 2020.

S. Alexandra, H. Vojtková, S. Nosalj, E. Piecková, H. Švehláková, L. Kraková, H. Drahovská, B. Stalmachová, K. Kučová, D. Pangallo, ‘Aspergillus niger Environmental Isolates and Their Specific Diversity Through Metabolite Profiling, Front. Microbiol., 2021.

M. A. Toma, K. H. M. N. H. Nazir, Md. M. Mahmud, P. Mishra, Md. K. Ali, A. Kabir ,Md. A. H. Shahid, M. P. Siddique, Md. A. Alim, 'Isolation and Identification of Natural Colorant Producing Soil-Borne Aspergillus niger from Bangladesh and Extraction of the Pigment', Foods., vol. 10, no. 6, 2021.

S.Tadahiro, M. Toyoda, 'Metal Ions in Activated Carbon Improve the Detection Efficiency of Aflatoxin-Producing Fungi'. Toxins., vol. 11, no. 3, pp. 140, 2019

V. Martin, H. Ingmer, 'Antibacterial and antifungal properties of resveratrol', International Journal of Antimicrobial Agents., vol. 53, pp. 716-723 , 2019.

S. D Kobayashi, M. N. DeLeo F.R, 'Neutrophils and Bacterial Immune Evasion, J Innate Immun., vol.10, pp.432–44. 2018.

Qing, Yun'an, Lin Cheng, Ruiyan Li, Guancong Liu, Yanbo Zhang, Xiongfeng Tang, Jincheng Wang, He Liu, Yanguo Qin, 'Potential antibacterial mechanism of silver nanoparticles and the optimization of orthopedic implants by advanced modification'. Int J Nanomedicine., vol. 13, pp. 3311–3327. 2018

Y. Zimeng, C. Zhang, Y. Ran, 'The effects of clioquinol in morphogenesis, cell membrane and ion homeostasis in Candida albicans'. BMC Microbiology., vol. 20, no. 165, 2020.




DOI: http://dx.doi.org/10.18517/ijaseit.12.4.15682

Refbacks

  • There are currently no refbacks.


Published by INSIGHT - Indonesian Society for Knowledge and Human Development