Evaluating the Potential of Liquid Smoke in Protecting Cocoa Beans against Storage Pest

- Sutrisno, Courage Yaw Krah, Idham Sakti Harahap, - Samsudin


Insect attack of produce in storage is a major challenge in postharvest handling and contributes to food waste and loss. Attempts to use synthetic chemicals to control this have generated other health and environmental problems. This study aimed to investigate the potential of pyrolyzed cocoa pod husk as a biopesticide (liquid Smoke (LS)) to protect cocoa beans against storage pests.  The study was set up in a Completely Randomized Design (CRD) with five treatments (10%; 20%; 50%; 100 % concentrations of LS and 0 %as control). These were applied to the outer surface of mini jute sacks containing cocoa beans.  A GCMS analysis of the LS detected 20 compounds. A repellence test of LS showed a very strong repellence effect, repelling almost 90% of the insect population. The feeding deterrence index also caused a reduction in bean damage from 22% in control to 7.65% for 100% LS-treated beans. Weight loss was reduced from 2.13% in control to 0.11 % in the sample treated with 100% LS. The LS treatment did not cause any significant change in the FFA and pH content of the beans. The organoleptic test also proved that LS treatment did not cause any substantial change in the flavor and overall taste and aroma acceptability. Therefore, the liquid smoke can be used for protecting cocoa beans by spraying on the outer part of jute sacks containing beans.


Cocoa beans; pyrolysis; liquid smoke; postharvest; storage pest; Corcyra cephalonica.

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C. Abballe et al., “Cocoa beans and derived products: Effect of processing on polycyclic aromatic hydrocarbons levels,” Lwt, vol. 135, no. November 2019, 2021, doi: 10.1016/j.lwt.2020.110019.

ICCO, “Quarterly Bulletin of Cocoa Statistcs,” Q. Bull. cocoa Stat., vol. XLIII, no. 0308–4469, pp. 20–29, 2017.

J. Neilson, A. Dwiartama, N. Fold, and D. Permadi, “Resource-based industrial policy in an era of global production networks: Strategic coupling in the Indonesian cocoa sector,” World Dev., vol. 135, p. 105045, 2020, doi: 10.1016/j.worlddev.2020.105045.

A. Gvozdjakova et al., “Cocoa consumption and prevention of cardiometabolic diseases and other chronic diseases,” Role Funct. Food Secur. Glob. Heal., pp. 317–345, 2018, doi: 10.1016/B978-0-12-813148-0.00019-0.

L. Barbosa-Pereira, O. Rojo-Poveda, I. Ferrocino, M. Giordano, and G. Zeppa, “Assessment of volatile fingerprint by HS-SPME/GC-qMS and E-nose for the classification of cocoa bean shells using chemometrics,” Food Res. Int., vol. 123, pp. 684–696, 2019, doi: 10.1016/j.foodres.2019.05.041.

A. Marseglia, M. Musci, M. Rinaldi, G. Palla, and A. Caligiani, “Volatile fingerprint of unroasted and roasted cocoa beans (Theobroma cacao L.) from different geographical origins,” Food Res. Int., vol. 132, p. 109101, 2020, doi: 10.1016/j.foodres.2020.109101.

G. M. I. Predan, A. L. Daniela, and I. L. Iulia, “Cocoa industry—from plant cultivation to cocoa drinks production,” in Caffeinated and Cocoa Based Beverages, vol. 8, 2019, pp. 489–507.

L. A. Domínguez-Pérez, L. M. Beltrán-Barrientos, A. F. González-Córdova, A. Hernández-Mendoza, and B. Vallejo-Cordoba, “Artisanal cocoa bean fermentation: From cocoa bean proteins to bioactive peptides with potential health benefits,” J. Funct. Foods, vol. 73, no. May, p. 104134, 2020, doi: 10.1016/j.jff.2020.104134.

M. S. Beg, S. Ahmad, K. Jan, and K. Bashir, “Status, supply chain and processing of cocoa - A review,” Trends Food Sci. Technol., vol. 66, pp. 108–116, Aug. 2017, doi: 10.1016/j.tifs.2017.06.007.

W. A. Jonfia-Essien, “Screening of new cocoa types for insect infestation and biochemical analysis of the stored beans,” Pakistan J. Biol. Sci., vol. 9, no. 14, pp. 2564–2571, 2006, doi: 10.3923/pjbs.2006.2564.2571.

K. D. Singh, A. J. Mobolade, R. Bharali, D. Sahoo, and Y. Rajashekar, “Main plant volatiles as stored grain pest management approach: A review,” J. Agric. Food Res., vol. 4, no. January, p. 100127, 2021, doi: 10.1016/j.jafr.2021.100127.

P. Agrafioti, C. G. Athanassiou, and M. K. Nayak, “Detection of phosphine resistance in major stored-product insects in Greece and evaluation of a field resistance test kit,” J. Stored Prod. Res., vol. 82, pp. 40–47, 2019, doi: 10.1016/j.jspr.2019.02.004.

M. S. Beg, S. Ahmad, K. Jan, and K. Bashir, “Status, supply chain and processing of cocoa - A review,” Trends in Food Science and Technology. 2017, doi: 10.1016/j.tifs.2017.06.007.

E. Teye, E. Anyidoho, R. Agbemafle, L. K. Sam-Amoah, and C. Elliott, “Cocoa bean and cocoa bean products quality evaluation by NIR spectroscopy and chemometrics: A review,” Infrared Phys. Technol., vol. 104, p. 103127, 2020, doi: 10.1016/j.infrared.2019.103127.

C. O. Jayeola, B. A. Adebowale, L. E. Yahaya, S. O. Ogunwolu, and O. Olubamiwa, “Production of Bioactive Compounds from Waste,” in Therapeutic, Probiotic, and Unconventional Foods, Elsevier, 2018, pp. 317–340.

J. I. B. Janairo and D. M. Amalin, “Volatile chemical profile of cacao liquid smoke,” Int. Food Res. J., vol. 25, no. 1, pp. 213–216, 2018.

S. Harti, A. Indriati, and S. Dyah, “Utilization of Liquid Smoke from Cocoa pod husk (Theobroma cocoa L) for Germination of Red Seed (Capsicum annum L),” Asian J. Appl. Sci., vol. 8, no. 1, pp. 172–184, Feb. 2020, doi: 10.24203/ajas.v8i1.6045.

N. Chaudhuri and S. K. Senapati, “Development and reproductive performance of rice moth Corcyra cephalonica Stainton (Lepidoptera: Pyralidae) in different rearing media,” J. Saudi Soc. Agric. Sci., vol. 16, no. 4, pp. 337–343, 2017, doi: 10.1016/j.jssas.2015.11.004.

J. Cheng, S. C. Hu, K. Kang, X. M. Li, Z. C. Geng, and M. Q. Zhu, “The effects of pyrolysis temperature and storage time on the compositions and properties of the pyroligneous acids generated from cotton stalk based on a polygeneration process,” Ind. Crops Prod., vol. 161, no. January, p. 113226, 2021, doi: 10.1016/j.indcrop.2020.113226.

C. Y. Krah, Sutrisno, Samsudin, and I. S. Harahap, “Use of liquid smoke for sustainable food preservation and postharvest loss and waste reduction (A review),” J. Appl. Phys. Sci., vol. 5, no. 2, Jun. 2019, doi: 10.20474/japs-5.2.1.

Y. X. Feng, Y. Wang, Z. F. Geng, D. Zhang, B. Almaz, and S. S. Du, “Contact toxicity and repellent efficacy of Valerianaceae spp. to three stored-product insects and synergistic interactions between two major compounds camphene and bornyl acetate,” Ecotoxicol. Environ. Saf., vol. 190, no. September 2019, p. 110106, 2020, doi: 10.1016/j.ecoenv.2019.110106.

V. Rajkumar, C. Gunasekaran, I. K. Christy, J. Dharmaraj, P. Chinnaraj, and C. A. Paul, “Toxicity, antifeedant and biochemical efficacy of Mentha piperita L. essential oil and their major constituents against stored grain pest,” Pestic. Biochem. Physiol., vol. 156, no. November 2018, pp. 138–144, 2019, doi: 10.1016/j.pestbp.2019.02.016.

G. Abdullahi, R. Muhamad, O. Dzolkhifli, and U. R. Sinniah, “Damage potential of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) on cocoa beans: Effect of initial adult population density and post infestation storage time,” J. Stored Prod. Res., vol. 75, pp. 1–9, 2018, doi: 10.1016/j.jspr.2017.11.001.

R. I. Urrutia, C. Yeguerman, E. Jesser, V. S. Gutierrez, M. A. Volpe, and J. O. Werdin González, “Sunflower seed hulls waste as a novel source of insecticidal product: Pyrolysis bio-oil bioactivity on insect pests of stored grains and products,” J. Clean. Prod., vol. 287, no. xxxx, p. 125000, Mar. 2021, doi: 10.1016/j.jclepro.2020.125000.

P. Mounjouenpou et al., “Temperature/duration couples’ variation of cocoa beans roasting on the quantity and quality properties of extracted cocoa butter,” Ann. Agric. Sci., vol. 63, no. 1, pp. 19–24, 2018, doi: 10.1016/j.aoas.2018.04.001.

H. S. adiah Abdul Halim, J. Selamat, S. H. Mirhosseini, and N. Hussain, “Sensory preference and bloom stability of chocolate containing cocoa butter substitute from coconut oil,” J. Saudi Soc. Agric. Sci., vol. 18, no. 4, pp. 443–448, 2019, doi: 10.1016/j.jssas.2018.02.005.

I. Ketut Budaraga and D. P. Putra, “Study of the physical properties of liquid smoke from cocoa rind on moisture content and different pyrolysis temperature,” IOP Conf. Ser. Earth Environ. Sci., vol. 542, no. 1, 2020, doi: 10.1088/1755-1315/542/1/012045.

Andy, E. Abustam, R. Malaka, and S. Purwanti, “A review of Encapsulated liquid smoke,” IOP Conf. Ser. Earth Environ. Sci., vol. 492, no. 1, 2020, doi: 10.1088/1755-1315/492/1/012061.

L. Handojo, Cherilisa, and A. Indarto, “Cocoa bean skin waste as potential raw material for liquid smoke production,” Environ. Technol. (United Kingdom), vol. 41, no. 8, pp. 1044–1053, 2020, doi: 10.1080/09593330.2018.1520306.

B. Babinszki et al., “Thermal decomposition of biomass wastes derived from palm oil production,” J. Anal. Appl. Pyrolysis, vol. 155, no. December 2020, 2021, doi: 10.1016/j.jaap.2021.105069.

R. V. S. Silva, V. B. Pereira, K. T. Stelzer, T. A. Almeida, G. A. Romeiro, and D. A. Azevedo, “Comprehensive study of the liquid products from slow pyrolysis of crambe seeds: Bio-oil and organic compounds of the aqueous phase,” Biomass and Bioenergy, vol. 123, no. February, pp. 78–88, 2019, doi: 10.1016/j.biombioe.2019.02.014.

G. Bonanomi et al., “Biochar-derived smoke-water exerts biological effects on nematodes, insects, and higher plants but not fungi,” Sci. Total Environ., vol. 750, p. 142307, 2021, doi: 10.1016/j.scitotenv.2020.142307.

S. Arivoli and S. Tennyson, “Antifeedant Activity of Plant Extracts Against Spodoptera litura (Fab.) (Lepidoptera: Noctuidae),” Am. J. Agric. Environ. Sci., vol. 12, no. 6, pp. 87–96, 2013, doi: 10.5829/idosi.aejaes.2012.12.06.63178.

E. Sapindal, K. H. Ong, and P. J. Hung King, “Efficacy of Azadirachta excelsa vinegar against Plutella xylostella,” Int. J. Pest Manag., vol. 64, no. 1, pp. 39–44, 2018, doi: 10.1080/09670874.2017.1293866.

J. Vachon et al., “Use of lignin as additive in polyethylene for food protection: Insect repelling effect of an ethyl acetate phenolic extract,” Compos. Part C Open Access, vol. 2, no. July, p. 100044, 2020, doi: 10.1016/j.jcomc.2020.100044.

M. A. Quelal-vásconez and É. Pérez-esteve, “Roadmap of cocoa quality and authenticity control in the industry: A review of conventional and alternative methods,” no. June 2019, pp. 448–478, 2020, doi: 10.1111/1541-4337.12522.

E. A. Oyewo and B. O. Amo, “Assessment of damage caused by Ephestia cautella (Walker) to stored cocoa beans,” Ghana J. Agric. Sci., vol. 52, no. 1, pp. 25–31, 2018, doi: 10.4314/gjas.v52i1.

A. Servent et al., “Assessment of cocoa (Theobroma cacao L.) butter content and composition throughout fermentations,” Food Res. Int., vol. 107, no. February, pp. 675–682, 2018, doi: 10.1016/j.foodres.2018.02.070.

V. Lemarcq et al., “Roasting-induced changes in cocoa beans with respect to the mood pyramid,” Food Chem., vol. 332, p. 127467, 2020, doi: 10.1016/j.foodchem.2020.127467.

B. Urbańska and J. Kowalska, “Comparison of the total polyphenol content and antioxidant activity of chocolate obtained from roasted and unroasted cocoa beans from different regions of the world,” Antioxidants, vol. 8, no. 8, 2019, doi: 10.3390/antiox8080283.

DOI: http://dx.doi.org/10.18517/ijaseit.11.6.14691


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