Preliminary Study of Ozonated Water to Preserve the Quality of Tofu: The Effect of Exposure Time and Replacement of Ozonated Water

Eva Fathul Karamah, Muhammad Zaki Zahirsyah, Rana Rezeki Najeges

Abstract


Tofu is a very popular source of vegetable protein in Indonesia. However, tofu is a very perishable food type because its water content is also very high and high in protein. Ozonated water is one of many solutions to preserve the quality of tofu. Ozone is an anti-microbial agent and is already recognized as safe to be contacted with food. This research aims to see the effect of exposure time and replacement of ozonated water in preserving tofu’s quality. The quality parameters that are observed in this research are total mesophilic aerobic bacteria (TMAB), pH, and protein level. The tofu is exposed to ozonated water (0.32 mg O3/L) for 40, 80, and 120 minutes. For water replacement, tofu is exposed to ozonated water (0.32 mg O3/L) for 120 minutes, and the water replacement occurred every 40 and 60 minutes (unreplaced water is observed as well). One hundred twenty minutes exposure can disinfect 51% TMAB and suppress the rate of change in pH and protein level in tofu. Moreover, water replacement every 40 minutes can disinfect 96% TMAB and suppress the rate of change of pH and protein level. This research shows that replacing ozonated water every 40 minutes is the most effective treatment among others in preserving the quality of tofu.

Keywords


Food quality; mesophyll-aerobic bacteria; ozonated water; ozone; tofu.

Full Text:

PDF

References


M. A. A. Mamun, M. A. Rahman, M. K. Zaman, Z. Ferdousi and M. A. Reza, “Toxicological effect of formalin as food preservative on kidney and liver tissues in mice model,†IOSR Journal of Environmental Science, Toxicology and Food Technology, Volume 8, Issue 9 Ver. II, pp. 47-5, Sep. 2014.

B. Maherani, M. Harich, S. Salmieri, and M. Lacroix, “Antibacterial Properties Of Combined non-thermal treatments based on bioactive edible coating, ozonation, and gamma irradiation on ready-to-eat frozen green peppers: evaluation of their freshness and sensory qualities,†European Food Research and Technology, vol. 245, pp. 1095–1111, 2019.

E.F. Karamah, S Z Adi, and N. Wajdi, “Effect of ozone exposure time and ozonated water replacement to control the quality of chicken meat,†Journal of Physics: Conference Series, Vol. 1295 no. 012068, 2019.

A.J. Brodowska, A. Nowak, and K. Śmigielski, “Ozone in the food industry: Principles of ozone treatment, mechanisms of action, and applications: An overview,†Critical reviews in food science and nutrition, pp.1-26, 2017.

E.F. Karamah, A.P. Ilmiyah, and N. Ismaningtyas, “The application of ozonated water to maintain the quality of tuna meat: the effect of contact time, contact temperature and ozone dosageâ€. IOP Conf. Ser.: Mater. Sci. Eng. Vol, 509, no. 012004, 2019.

A. Alexopoulos, S. Plessas, Y. Kourkoutas, C. Stefanis, S. Vavias, and C. Voidarou, “Experimental effect of ozone upon the microbial flora of commercially produced dairy fermented products,†International Journal of Food Microbiology, vol. 246, pp. 5–11, 2017.

R. Aslam, M.S. Alam, and P.A. Saeed, “Sanitization Potential of Ozone and Its Role in Postharvest Quality Management of Fruits and Vegetables,†Food Engineering Reviews, vol. 12, pp. 48–67, 2020.

S.J._e Granella, D. Christ, I. Werncke, T.R. Bechlin and S.R.M. Coelho, “Effect of drying and ozonation process on naturally contaminated wheat seeds,†Journal of Cereal Science, vol. 80, pp. 205-211, 2018.

S.T. Moraglio, L. Bosco, C. Pogolotti, L. Tavella, “Effect of ozone gas against life stages of Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) in laboratory and a storehouse,†Journal of Stored Products Research, vol. 79, pp. 132-138, 2018.

M. Aponte, A. Anastasio, R. Marrone, R. Mercogliano, M.F. Peruzy, N. Murru, “Impact of gaseous ozone coupled to passive refrigeration system to maximize shelf-life and quality of four different fresh fish products,†Food Science and Technology, vol. 93, pp. 412–419, 2018.

Z. Zhou, S. Zuber, F. Cantergiani, I. Sampers, F. Devlieghere, and M. Uyttendaele, “Inactivation of Foodborne Pathogens and Their Surrogates on Fresh and Frozen Strawberries Using Gaseous Ozone,†Frontiers in Sustainable Food Systems, vol. 2, p.51, 2018.

B. Gim´enez, N. Graiver, L. Giannuzzi, and N. Zaritzky, “Treatment of beef with gaseous ozone: Physicochemical aspects and anti-microbial effects on heterotrophic microflora and listeria monocytogenes,†Food Control, vol. 121, no. 107602, pp. 1-9, 2021.

A. Baggio, · M. Marino, · N. Innocente, · M. Celotto, · and M. Maifreni, “Anti-microbial effect of oxidative technologies in food processing: an overview,†European Food Research and Technology, vol. 246, pp. 669–692, 2020.

E.F. Karamah, R.R. Najeges, M.Z. Zahirsyah, “The influence of ozone dosage, exposure time and contact temperature of ozone in controlling food quality (case study: tofu),†IOP Conf. Series: Materials Science and Engineering, vol. 509, no. 012117, 2019.

O'Donnell, C., Tiwari, B.K., Cullen, P.J. and Rice, R.G. eds., (2012). Ozone in food processing. John Wiley & Sons.

Kleiner, I.S. dan J.M. Orten. (1975). Biochemistry. New York: The C.V. Mosby Co.

A. Baggio, M. Marino, N. Innocente, M. Celotto, and M. Maifreni, “Anti-microbial effect of oxidative technologies in food processing: an overview,†European Food Research and Technology, vol. 246, pp. 669–692, 2020.

E.F. Karamah, N. Ismaningtyas, and A.P. Ilmiyah. “The effect of exposure time and water replacement in the application of ozonated water to maintain the quality of tunaâ€. IOP Conf. Ser.: Mater. Sci. Eng. Vol, 509, no. 012092, 2019.

S. Shezi, L.S. Magwaza, A. Mditshwa, and S.Z. Tesfay,†Review. Changes in biochemistry of fresh produce in response to ozone postharvest treatment,†Scientia Horticulturae, vol. 269, pp.1-9, 2020.

E.F. Karamah, N. Ismaningtyas, and A.P. Ilmiyah. “The effect of exposure time and water replacement in the application of ozonated water to maintain the quality of tunaâ€. IOP Conf. Ser.: Mater. Sci. Eng. Vol, 509, no. 012092, 2019.




DOI: http://dx.doi.org/10.18517/ijaseit.11.3.13130

Refbacks

  • There are currently no refbacks.



Published by INSIGHT - Indonesian Society for Knowledge and Human Development