The Evaluation of Frequencies of Cytogenetic Biomarkers in Lymphocyte of Residents from High Natural Radiation Area in West Sulawesi, Indonesia

Mukh Syaifudin, Dwi Ramadhani, Sofiati Purnami, Nastiti Rahajeng, Siti Nurhayati, Harry Nugroho Eko Surniyantoro

Abstract


Residents living in high natural radiation areas may pose health consequences such as cancer. The study aims to evaluate the frequencies of cytogenetic biomarkers covering micronuclei (MN), nucleoplasmic bridge (NPB), and nuclear bud (NBUD) with the cytokinesis-block micronucleus (CBMN) assay. This cross-sectional study was done on 51 blood lymphocytes from the resident of Mamuju, West Sulawesi, Indonesia that was done according to standard procedure. After being stained with Giemsa solution, these biomarkers were observed on about 1,000 binucleated cells. The results showed a low frequency of MN (0.0162 in 36,091 cells) and extremely low frequencies of other biomarkers (0.00019 and 0.00061 for NPB and NBUD, respectively) in the study area, whereas these were 0.0225 MNs in 15,000 cells, and 0.00013 and 0.00120 for NPB and NBUD, respectively, in the control area. MNs and NBUDs were lower in the study area compared to control. No statistically significant differences (p>0.05) in NPB were found between the two areas, but not for NBUD. The frequencies of MN and NPB in female is higher than that of male in both areas. In the control group, males experienced a decrease in the number of NPB to 0.7 times compared to females, and every extra one year of age, 1.047 times more NBUDs were found. None of the confounding factors was influenced in the study group. It was concluded that there is no impact of high natural radiation to the local residents based on cytogenetics evaluation, with a note that further studies on a higher number of samples and other relevant biomarkers are required.

Keywords


Natural radiation; cytogenetic biomarkers; MN; NPB; Mamuju.

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References


D. Zeegers et al., “Biomarkers of ionizing radiation exposure: A multiparametric approach,” Genome Integr., vol. 8, no. 1, pp. 1–6, 2017, doi: 10.4103/2041-9414.198911.

A. Baeza, J. García-Paniagua, J. Guillén, and B. Montalbán, “Influence of architectural style on indoor radon concentration in a radon prone area: A case study,” Sci. Total Environ., vol. 610–611, pp. 258–266, 2018, doi: 10.1016/j.scitotenv.2017.08.056.

A. V. Akleyev, “Normal tissue reactions to chronic radiation exposure in man,” Radiat. Prot. Dosimetry, vol. 171, no. 1, pp. 107–116, 2016, doi: 10.1093/rpd/ncw207.

M. Ricoul, T. Gnana-Sekaran, L. Piqueret-Stephan, and L. Sabatier, “Cytogenetics for Biological Dosimetry.,” Methods Mol. Biol., vol. 1541, pp. 189–208, 2017, doi: 10.1007/978-1-4939-6703-2_17.

S. Sommer, I. Buraczewska, and M. Kruszewski, “Micronucleus assay: The state of art, and future directions,” Int. J. Mol. Sci., vol. 21, no. 4, pp. 7–9, 2020, doi: 10.3390/ijms21041534.

H. S. J. Cheong, I. Seth, M. C. Joiner, and J. D. Tucker, “Relationships among micronuclei, nucleoplasmic bridges and nuclear buds within individual cells in the cytokinesis-block micronucleus assay,” Mutagenesis, vol. 28, no. 4, pp. 433–440, 2013, doi: 10.1093/mutage/get020.

M. Kirsch-Volders, M. Fenech, and C. Bolognesi, “Validity of the Lymphocyte Cytokinesis-Block Micronucleus Assay (L-CBMN) as biomarker for human exposure to chemicals with different modes of action: A synthesis of systematic reviews.,” Mutat. Res. Genet. Toxicol. Environ. Mutagen., vol. 836, no. Pt A, pp. 47–52, Dec. 2018, doi: 10.1016/j.mrgentox.2018.05.010.

B. Ruiz-Ruiz et al., “Cytokinesis-Block Micronucleus Assay Using Human Lymphocytes as a Sensitive Tool for Cytotoxicity/Genotoxicity Evaluation of AgNPs,” ACS Omega, vol. 5, no. 21, pp. 12005–12015, 2020, doi: 10.1021/acsomega.0c00149.

A. Nersesyan et al., “Use of the lymphocyte cytokinesis-block micronucleus assay in occupational biomonitoring of genome damage caused by in vivo exposure to chemical genotoxins: Past, present and future,” Mutat. Res. - Rev. Mutat. Res., vol. 770, pp. 1–11, 2016, doi: 10.1016/j.mrrev.2016.05.003.

M. A. Rodrigues, L. A. Beaton-Green, R. C. Wilkins, and M. F. Fenech, “The potential for complete automated scoring of the cytokinesis block micronucleus cytome assay using imaging flow cytometry,” Mutat. Res. - Genet. Toxicol. Environ. Mutagen., vol. 836, no. May, pp. 53–64, 2018, doi: 10.1016/j.mrgentox.2018.05.003.

M. Salimi and H. Mozdarani, “Different aspects of cytochalasin B blocked micronucleus cytome (CBMN cyt) assay as a comprehensive measurement tool for radiobiological studies, biological dosimetry and genome instability,” Int. J. Radiat. Res., vol. 13, no. 2, pp. 101–126, 2015, doi: 10.7508/ijrr.2015.02.001.

A. Podrimaj-Bytyqi, A. Borovečki, Q. Selimi, S. Manxhuka-Kerliu, G. Gashi, and I. R. Elezaj, “The frequencies of micronuclei, nucleoplasmic bridges and nuclear buds as biomarkers of genomic instability in patients with urothelial cell carcinoma,” Sci. Rep., vol. 8, no. 1, pp. 1–9, 2018, doi: 10.1038/s41598-018-35903-5.

V. Kravtsov, A. Livanova, O. Belyakov, and R. Fedortseva, “The frequency of lymphocytes containing dumbbell-shaped nuclei depends on ionizing radiation dose and correlates with appearance of chromosomal aberrations,” Genome Integr., vol. 9, no. 1, pp. 1–7, 2018, doi: 10.4103/genint.genint_1_18.

M. Syaifudin, V. Defiyandra, S. Nurhayati, S. Purnami, and E. Pudjadi, “Micronucleus assay-based evaluation of radiosensitivity of lymphocytes among inhabitants living in high background radiation area of Mamuju, West Sulawesi, Indonesia,” Genome Integr., vol. 9, no. 1, pp. 1–5, 2018, doi: 10.4103/genint.genint_2_18.

M. Syaifudin et al., “Cytogenetic and Molecular Damages in Blood Lymphocyte of Inhabitants Living in High Level Natural Radiation Area (HLNRA) of Botteng Village, Mamuju, West Sulawesi, Indonesia,” J. Radiat. Environ. Med., vol. 7, no. 2, pp. 65–76, 2018.

T. Leshukov, A. Larionov, K. Legoshchin, and Y. Lesin, “The Assessment of Radon Emissions as Results of the Soil Technogenic Disturbance.”

D. Ramadhani et al., “Assessment of Individual Radiosensitivity in Inhabitants of Takandeang Village - A High Background Radiation Area in Indonesia,” Atom Indones., vol. 45, no. 1, pp. 27–35, 2019, doi: 10.17146/aij.2019.724.

R. I. Bersimbaev and O. Bulgakova, “The health effects of radon and uranium on the population of Kazakhstan,” Genes Environ., vol. 37, no. 1, pp. 1–10, 2015, doi: 10.1186/s41021-015-0019-3.

B. Baselet, C. Rombouts, A. M. Benotmane, S. Baatout, and A. Aerts, “Cardiovascular diseases related to ionizing radiation: The risk of low-dose exposure (Review),” Int. J. Mol. Med., vol. 38, no. 6, pp. 1623–1641, 2016, doi: 10.3892/ijmm.2016.2777.

I. A. E. Agency, “Cytogenetic Dosimetry : Applications in Preparedness for and Response to Radiation Emergencies,” Man. Ser., p. 247, 2011.

S. Knasmüller and M. Fenech, The Micronucleus Assay in Toxicology. The Royal Society of Chemistry, 2019.

P. Thomas and M. Fenech, “Cytokinesis-Block Micronucleus Cytome Assay in Lymphocytes BT - DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols,” V. V Didenko, Ed. Totowa, NJ: Humana Press, 2011, pp. 217–234.

S. Anbumani and M. N. Mohankumar, “Nucleoplasmic bridges and tailed nuclei are signatures of radiation exposure in Oreochromis mossambicus using erythrocyte micronucleus cytome assay (EMNCA).,” Environ. Sci. Pollut. Res. Int., vol. 22, no. 23, pp. 18425–18436, Dec. 2015, doi: 10.1007/s11356-015-5107-1.

S. Purnami, M. Lubis, S. Nurhayati, and D. Ramadhani, “Micronucleus Frequencies in Mononucleated Cells of People Living in Takandeang Village – A High Level of Natural Radiation Area in Indonesia Frekuensi Mikronukleus pada Sel Mononukleat Penduduk Desa Takandeang Daerah Radiasi Latar Tinggi di Indonesia,” J. Ilm. Apl. Isot. dan Radiasi, vol. 14, no. 2, pp. 117–124, 2018.

I. Dadong and S. Bunawas, “Mapping radiation and radioactivity in Sulawesi island,” 2010.

H. Syaeful, I. G. Sukadana, and A. Sumaryanto, “Radiometric mapping for Naturally Occurring Radioactive Materials (NORM) assessment in Mamuju, West Sulawesi,” Atom Indones., vol. 40, no. 1, pp. 33–39, 2014, doi: 10.17146/aij.2014.263.

B. Pardini et al., “Increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of bladder cancer,” Br. J. Cancer, vol. 116, no. 2, pp. 202–210, 2017, doi: 10.1038/bjc.2016.411.

A. Zeller et al., “A critical appraisal of the sensitivity of in vivo genotoxicity assays in detecting human carcinogens,” Mutagenesis, vol. 33, no. 2, pp. 179–193, 2018, doi: 10.1093/mutage/gey005.

S. Pfuhler et al., “Use of in vitro 3D tissue models in genotoxicity testing : Strategic fit , validation status and way forward . Report of the working group from the 7 th International Workshop on Genotoxicity Testing ( IWGT ),” Mutat Res Gen Tox En, vol. 850–851, no. January, p. 503135, 2020, doi: 10.1016/j.mrgentox.2020.503135.

X. Zhang et al., “Increased micronucleus, nucleoplasmic bridge, and nuclear bud frequencies in the peripheral blood lymphocytes of diesel engine exhaust-exposed workers,” Toxicol. Sci., vol. 143, no. 2, pp. 408–417, 2015, doi: 10.1093/toxsci/kfu239.

X.-L. Tian et al., “Dose-effect relationships of nucleoplasmic bridges and complex nuclear anomalies in human peripheral lymphocytes exposed to 60Co γ-rays at a relatively low dose.,” Mutagenesis, vol. 31, no. 4, pp. 425–431, Jul. 2016, doi: 10.1093/mutage/gew001.

D. Zeljezic, M. Bjelis, and M. Mladinic, “Evaluation of the mechanism of nucleoplasmic bridge formation due to premature telomere shortening in agricultural workers exposed to mixed pesticides: Indication for further studies,” Chemosphere, vol. 120, pp. 45–51, 2015, doi: 10.1016/j.chemosphere.2014.05.085.

T.-J. Cai et al., “Effects of age and gender on the baseline and 2 Gy 60Co γ-ray-induced nucleoplasmic bridges frequencies in the peripheral blood lymphocytes of Chinese population,” Mutat. Res. Genet. Toxicol. Environ. Mutagen., vol. 832–833, p. 29—34, Aug. 2018, doi: 10.1016/j.mrgentox.2018.06.013.

H. N. E. Surniyantoro, Y. Lusiyanti, T. U. R. Rahardjo, S. Nurhayati, and D. Tetriana, “Association between XRCC1 exon 10 (Arg399Gln) gene polymorphism and micronucleus as a predictor of DNA damage among radiation workers,” Biodiversitas, vol. 19, no. 5, pp. 1676–1682, 2018, doi: 10.13057/biodiv/d190512.

G. Gashi, V. Mahovlić, S. Manxhuka-Kerliu, A. Podrimaj-Bytyqi, L. Gashi, and I. R. Elezaj, “The association between micronucleus, nucleoplasmic bridges, and nuclear buds frequency and the degree of uterine cervical lesions.,” Biomarkers Biochem. Indic. Expo. response, susceptibility to Chem., vol. 23, no. 4, pp. 364–372, 2018, doi: 10.1080/1354750X.2018.1428828.

D. Kumar et al., “Genetic Instability in Lymphocytes is Associated with Blood Plasma Antioxidant Levels in Health Care Workers Occupationally Exposed to Ionizing Radiation,” Int. J. Toxicol., vol. 35, no. 3, pp. 327–335, 2016, doi: 10.1177/1091581815625593.

N. Nakamura, “Why Genetic Effects of Radiation are Observed in Mice but not in Humans.,” Radiat. Res., vol. 189, no. 2, pp. 117–127, Feb. 2018, doi: 10.1667/RR14947.1.

J.-I. Asakawa et al., “Genome-Wide Deletion Screening with the Array CGH Method in Mouse Offspring Derived from Irradiated Spermatogonia Indicates that Mutagenic Responses are Highly Variable among Genes.,” Radiat. Res., vol. 186, no. 6, pp. 568–576, Dec. 2016, doi: 10.1667/RR14402.1.

Moshupya, Abiye, H. Mouri, Levin, Strauss, and Strydom, “Assessment of Radon Concentration and Impact on Human Health in a Region Dominated by Abandoned Gold Mine Tailings Dams: A Case from the West Rand Region, South Africa,” Geosciences, vol. 9, p. 466, Oct. 2019, doi: 10.3390/geosciences9110466.




DOI: http://dx.doi.org/10.18517/ijaseit.12.1.12665

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