Isotopic Tracing of Phosphorus Uptake in Oil Palm Seedlings Leaf Axil Using 32P Labelled

Warmanti Mildaryani, Mujiyo Mujiyo, Widyatmani Sih Dewi, Djoko Poernomo

Abstract


Pot experiments have been carried out to investigate the distribution pattern of Phosphorus in oil palm seedlings axillary axes using 32P radioisotope technique. The experiment was conducted in the greenhouse at the Center for Application of Isotope and Radiation – National Nuclear Energy Agency, Jakarta, Indonesia. KH232PO4 solution with the activity of 5 mCi was injected into five points viz through the lowest, middle, upper leaf axil, at the base of the trunk and the soil 5 cm from the trunk. The method used was  randomized complete block design with two replications.The 32P uptake is expressed in counted per minute (cpm) counted by Liquid Scintillation Counter  then transformed to disintegration per minute (dpm). The results showed that at the injection point in the lowest leaf axil, the distribution of 32P was more towards the crown before heading towards the roots.In the middle of the leaf axil,the distribution of 32P was recordedhigher, nutrients were actively distributed to the top of the plant while the roots were very low. The dynamics of 32P at the upper axil injection point, the highest 32P uptake at the start of the injection was seen in the lowest leaf axil. The 32P distribution then leads to the stem, roots and finally to the canopy. At the injection point in the soil 5 cm from the trunk, the counted value shows 32P distributed to the top of the plant, while at the base of the trunk, 32P is distributed to the farthest injection point, the plant canopy. From this study,  it can be concluded that 32P radioisotopes can describe the distribution of nutrients in plant parts, wherever radioisotope is applied. Phosphorus can be applied through any leaf axil and can be distributed well to the canopy of plants.


Keywords


distribution of phosphorus; leaf axil; oil palm; radioisotop 32P

Full Text:

PDF

References


Safuan, La Ode; Fransiscus S. Rembon, dan Hasbullah Syaf1).†Evaluasi status hara tanah dan jaringan sebagai dasar rekomendasi pemupukan N, P, dan K pada tanaman kelapa sawitâ€. AGRIPLUS, Vol. 23 No. 02, Mei .pp. 154-162,2013, ISSN 0854-0128

Behera, BC, Singdevsachan, SK, Mishra, RR, Sethi, BK, Dutta, SK & Thatoi, HN, , “Phosphate Solubilising Bacteria from Mangrove Soils of Mahanadi River Delta, Odisha, India,â€World Journal of Agricultural Research, vol. 4, no. 1, pp. 18-23,2016

Broschat,Timothy K.â€Uptake and distribution of Boron in Coconut and paurotis palmsâ€. Hort Science vol.46 (12).pp.1683-1686, 2011

Maryani, A.T.. “Efek Pemberian Decanter Solid terhadap Pertumbuhan Bibit Kelapa Sawit (Elaeis guineensis Jacq) dengan Media Tanah Bekas Lahan Tambang Batu Bara di Pembibitan Utamaâ€. Caraka Tani: Journal of Sustainable Agriculture. 33(1),pp.50-56, 2018.

Tampubolon, K., Purba, E., Hanafiah, D. S., and Basyuni, M. “Sebaran Populasi dan Klasifikasi Resistensi Eleusine indica terhadap Glifosat pada Perkebunan Kelapa Sawit di Kabupaten Deli Serdangâ€. Caraka Tani: Journal of Sustainable Agriculture. 33(2),pp.146-152,2018.

Panggabean, S. Manahan; Purwono.†Manajemen Pemupukan Tanaman Kelapa Sawit (Elaeis Guineensis Jacq.) Di Pelantaran Agro Estate,Kalimantan Tengahâ€. Bul. Agrohorti 5 (3).pp. 316-324, 2017.

Rajaratnam,J.A.â€Application, absorption and translocation of Boron in oil palm. I. Method of application and types of boron fertilizerâ€.Expl.Agric. 9.pp.129-139, 1973

Claryssa M.Monteiro, Ediane S. Caron, Silvaldo F.da Silveira; Alexandre M.Almeida; Gilberto R.Souza-Filho; AleomarL.de Souza. “Control of foliar diseases by axillary application of systemic fungicides in Brazilian coconut palmsâ€. Crop Protection vol.52.pp.78-83, 2013

Zhang, Zhaoliang; Hong Liao and William J.Lucas. “Molecular mechanisms underlying phosphate sensing, signaling, and adaptation in plants. JIPB vol.56.pp 192-220, 2014

Cimpeanu, C.; C Barna; M. Iliescu. “32P- Radioactive tracer for the evaluation of fertilizer influence on nutrients translocation process from soil to the plantsâ€.Rom.Journ.Phys.,vol.59 Nov.pp.1048-1056,2014.

Citraresmini1,A., I. Anas and Nurmayulis.†The use of 32P Method to Evaluate the Growth of Lowland Rice Cultivated in a System of Rice Intensification (SRI)â€. Atom Indonesia Vol. 39 No. 2.pp. 88-94,2013.

Flatian, Anggi Nico; Sudono Slamet; dan Ania Citraresmini. “Perunutan Serapan Fosfor (P) Tanaman Sorgum Berasal dari 2 Jenis Pupuk yang Berbeda Menggunakan Teknik Isotop (32P)â€. Jurnal Ilmiah Aplikasi Isotop dan Radiasi. Vol. 14 No. 2. Desember.pp.109-115. 2018.

Flatian, Anggi Nico; Iswandi Anas;Atang Sutandi, Ishak. “Kontribusi P Berasal dari Aktivitas Mikrobia Pelarut Fosfat, Fosfat Alam dan SP-36 yang Ditentukan Menggunakan Teknik Isotop 32Pâ€.A Scientific Journal for The Applications of Isotope and Radiation. Vol.2 No.1 Juni.57-68, 2016.

Suyono, A.D. and A. Citraresmini. “Measurement of P Contribution From several P Sources by Using 32P Methodâ€.Atom Indonesia Vol.36 No.2 August. 69-75, 2010.

Shintarika ,Feni; Sudradjat; dan Supijatno.†Optimasi Dosis Pupuk Nitrogen dan Fosfor pada Tanaman Kelapa Sawit (Elaeis guineensis Jacq.) Belum Menghasilkan Umur Satu Tahunâ€. J. Agron. Indonesia 43 (3).pp. 250 – 256, 2015.

Suyono, A.D. and A. Citraresmini. “Measurement of P Contribution From several P Sources by Using 32P Methodâ€.Atom Indonesia Vol.36 No.2 August. 69-75, 2010.

IAEA.†Use of Phosphorus Isotopes for Improving Phosphorus Management in Agricultural Systemsâ€. International Atomic Energy Agency, Series: IAEA TECDOC series, ISSN 1011–4289 ; no. 1805,2016.

Taiz, Lincoln; Eduardo Zeiger; Ian Max Moller; Angus Murphy.â€Plant Physiology and Developmentâ€. Sinauer Associates. ISBN: 9781605352558; 761. 2015

Koontz,Harold and Orlin Biddulph. “Factor affecting absorption and translocation of foliar applied phosphorusâ€.Plant Physiology.pp.463-470.

Fernandez, V; Paula Guzman; Courtney A.E.Pierce; Therese M.McBeath; Mohamed Khayet; Mike J.McLaughlin . “Effect of wheat phosphorus status on leaf surface properties and permeability to foliar-applied phosphorusâ€.Plant and Soil.pp:1-22. 2014.

Sisworo,E.L.;K.Idris, A.Citraresmini and I.Sugoro.â€Nuclear Technique for Research on Soil-Plant Relations, Data Analysis and Interpretation, BATAN, Jakarta.2006.

Sutapa, Gusti Ngurah; Ni Nyoman Ratini, Gde Antha Kasmawan. 2016. “Analisis Waktu Pemupukan Tanaman Sawi Hijau (Brassica rapa var.parachinensis) dengan Teknik Perunut Radioaktif.†Jurnal Biologi Vol.20, No.1, Juni.pp : 35-39,2016.

G.J. Blair; J.J.Adu-Gyamfi and F. Zapata.â€Phosphorus Isotope Tracer Techniques Procedures and Safety Issues, in Use of Phosphorus Isotopes for Improving Phosphorus Management in Agricultural Sytemsâ€. IAEA Tecdoc Series, International Atomic Energy Agency, Vienna,pp.50-91, 2010.

Mohidin Hasmah ;Mohamed Musa Hanafi ; Yusop Mohd Rafii); Siti Nor Akmar Abdullah ; Abu Seman Idris ; Sulaiman Man; Juferi Idris ; Mahbod Sahebi. “Determination of optimum levels of nitrogen, phosphorus and potassium of oil palm seedlings in solution culturâ€. Bragantia Campinas.February.2015.




DOI: http://dx.doi.org/10.18517/ijaseit.10.1.11119

Refbacks

  • There are currently no refbacks.



Published by INSIGHT - Indonesian Society for Knowledge and Human Development